A0847

Adenylyl Cyclase Toxin from Bordetella pertussis

≥70% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, ≥50 units/mg protein

• UNSPSC Code: 41106305
• MDL number: MFCD00675046
• eCl@ss: 32110600

Propiedades

• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥70% (SDS-PAGE)
• form: lyophilized powder
• specific activity: ≥50 units/mg protein
• mol wt: 177 kDa by amino acid sequence
• storage temp.: 2-8°C
• Gene Information: human ... CAMP(820) ; mouse ... CAMP(12796) ; rat ... CAMP(316010)

Descripción

Biochem/physiol Actions

Adenylate cyclase toxin from Bordetella pertussis belongs to the family of pore-forming toxins. It converts host ATP to cyclic AMP (cAMP). May be used to affect intracellular cAMP levels in mammalian cells.

Preparation Note

When reconstituted with 50 μL of water, the protein is in 0.05 M Tris, 8 M urea, 0.002 M EDTA, pH 8.0.

Other Notes

One unit will produce 1.0 μmol cAMP from ATP per minute at pH 8.0 at 30 °C

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 2
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, type N95 (US)