A0924

Acrylamide/Bis-acrylamide

BioReagent, Molecular Biology, 49:1 (ratio)

• Sinónimos: Acrylamide/Bisacrylamide, 49:1 (2.0% crosslinker), Polyacrylamide solution
• NACRES: NA.25
• PubChem Substance ID: 329770639
• UNSPSC Code: 41105319
• MDL number: MFCD00080848
• eCl@ss: 32110600

Propiedades

• grade: Molecular Biology
• Quality Segment: 200
• product line: BioReagent
• form: powder
• feed ratio: 49:1 (ratio)
• foreign activity: DNase (Exonuclease), NICKase (Endonuclease), RNase, protease, none detected
• SMILES string: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• InChI key: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Descripción

Application

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Acrylamide/Bis-acrylamide has been used in the electrophoresis of SSCP (single-strand conformation)/heteroduplex.

Biochem/physiol Actions

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Preparation Note

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

To make up 100 ml of A0924, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Información de seguridad

• Pictogramas: GHS06,GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H301,H312 + H332,H315,H317,H319,H340,H350,H361fd,H372
• Indicaciones de precaución: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Órganos diana: Peripheral nervous system
• Clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• ¿Qué está pasando?: WGK 3
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges