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A2230

Sigma-Aldrich

Apyrase from potatoes

High Activity, ATPase ≥600 units/mg protein, lyophilized powder

Synonym(s):
Adenosine 5′-triphosphatase, Adenosine 5′-diphosphatase
CAS Number:
Enzyme Commission number:
Número MDL:
NACRES:
NA.54

Nivel de calidad

300

origen biológico

potato

formulario

lyophilized powder

calidad

High Activity

ATPase activity

≥600 units/mg protein

secondary activity

≥50 % of base activity ADPase

composición

protein, ≥30%

actividad extraña

Acid Phosphatase ≤2% of base activity

enviado en

wet ice

temp. de almacenamiento

−20°C

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Aplicación

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation . Product A2230 is a high activity apyrase.
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Envase

100 units in glass bottle
Sold on the basis of ATPase units.

Acciones bioquímicas o fisiológicas

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Definición de unidad

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Forma física

Lyophilized powder containing potassium succinate buffer salts.

Nota de preparación

Derived from red potato

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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