A6691

Amidase from Pseudomonas aeruginosa

recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)

• Sinónimos: Acrylamide Amidohydrolase, Acylase
• Número CAS: 9012-56-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• Número CE: 3.5.1.4 (BRENDA, IUBMB)
• MDL number: MFCD00166319
• eCl@ss: 32160703
• Recombinant: expressed in E. coli
• Concentration: 14 mg/mL

Propiedades

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• hydroxamate transferase activity: ≥200 units/mg protein (biuret)
• concentration: 14 mg/mL
• UniProt accession no.: Q9HWL8
• storage temp.: −20°C
• Gene Information: Pseudomonas aeruginosa PAO1 ... PA4163(880181)

Descripción

Application

The importance of these hydrolases in biotechnology is growing rapidly, because their potential applications span through chemical and pharmaceutical industries as well as in bioremediation. Immobilized amidase can be used efficiently for production of acrylic acid from acrylamide, thus converting a toxic ambient contaminant into widely used industrial raw material. Amidases are potential treatments for human immunodeficiency virus and malaria. They may be used to eliminate metal ions in wastewater .

Amidase from Pseudomonas aeruginosa has been used for testing its capability to hydrolyze ochratoxin A.

Biochem/physiol Actions

The amidase from Pseudomonas aeruginosa isa 6 × 38-kDa enzyme that catalyzes the hydrolysis of a small range of short aliphatic amides. Each amidase monomer is formed by a globular four-layer αββα sandwich domain with an additional 81-residue long C-terminal segment .

Physical form

Solution in 50% glycerol containing 7 mM 2-mercaptoethanol and phosphate buffer salt

Other Notes

One unit will convert 1.0 μmole of acetamide and hydroxylamine to acetohydroxamate and ammonia per min at pH 7.2 at 37 °C.

Información de seguridad

• Pictogramas: GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H334
• Indicaciones de precaución: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)