A9972

α-Amylase from human pancreas

lyophilized powder, ≥100 units/mg protein

• Número CAS: 9000-90-2
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-565-6
• NACRES: NA.54
• MDL number: MFCD00081319
• Número CE: 3.2.1.1 (BRENDA, IUBMB)

Propiedades

• biological source: human pancreas
• form: lyophilized powder
• specific activity: ≥100 units/mg protein
• purified by: 2× crystallization
• sustainability: Sustainability features
• UniProt accession no.: P04746, P19961
• storage temp.: −20°C
• Gene Information: human ... AMY1A(279), AMY2B(280)

Descripción

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Biochem/physiol Actions

α-Amylase is composed of 496 amino acids in a single polypeptide chain. It hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.

Physical form

Lyophilized from Tris buffer containing NaCl and CaCl₂.

Preparation Note

Prepared by modified method of Levitzki et al.

Other Notes

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Información de seguridad

• Pictogramas: GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H334
• Indicaciones de precaución: P261 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 13 - Non Combustible Solids
• ¿Qué está pasando?: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable