AP0100

Sigma-Aldrich

Alkaline Phosphatase Diethanolamine Activity Kit

sufficient for 100 tests

NACRES:
NA.26

Nivel de calidad

200

uso

sufficient for 100 tests

temp. de almacenamiento

2-8°C

Descripción general

The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.

Aplicación

Alkaline Phosphatase Diethanolamine Activity Kit has been used:
  • in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts
  • for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells
  • for determining ALP activity in the bone metastasis model of lung cancer
  • for measuring ALP levels in colon tissue homogenates

Idoneidad

Suitable for the measurement of alkaline phosphatase activity in a variety of samples

Principio

Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • Alkaline Phosphatase Diethanolamine Reaction Buffer

  • P4744Phosphatase substrate, powder

  • P6774Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous solution, ≥2,000 DEA units/mg protein

pictogramas

CorrosionHealth hazard

Palabra de señalización

Danger

Frases de peligro

Consejos de prudencia

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

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