C0979

Sigma-Aldrich

Anti-Catalase antibody ,Mouse monoclonal

clone CAT-505, purified from hybridoma cell culture

Número MDL:

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

purified from hybridoma cell culture

antibody product type

primary antibodies

clon

CAT-505, monoclonal

formulario

buffered aqueous solution

mol peso

antigen 60 kDa

species reactivity

bovine, human, mouse, rat

concentración

~2 mg/mL

aplicaciones

immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using a whole extract of HepG2 cells

isotipo

IgG1

conjugado

unconjugated

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... CAT(847)
mouse ... Cat(12359)
rat ... Cat(24248)

Descripción general

Catalase is one of the most efficient and common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). In most mammalian cell types catalase is localized in peroxisomes which is a tertrameric haemin-enzyme consisting of 4 identical tetrahedrally arranged subunits of 60kDa. It contains four ferriprotoporphyrin groups per molecule having a molecular mass of 240kDa.

Inmunógeno

human erythrocyte catalase

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (5 papers)
Monoclonal Anti-Catalase antibody produced in mouse is suitable for the following:
  • Immunocytochemistry
  • Immunohistochemistry
  • Indirect ELISA
  • Microarray
  • Western blotting at a concentration of 0.5-1μg/mL using whole extract of HepG2 cells
  • Immunofluorescence

Acciones bioquímicas o fisiológicas

Catalase reacts with hydrogen peroxide, a reactive oxygen species, activating its decomposition into water and molecular oxygen and it reacts with hydrogen donors. It functions as a natural anti-oxidant, protecting cells against oxidative damage of proteins, lipids and nucleic acids. Catalase also plays a role in gene expression and apoptosis. Antioxidants have been identified in association with a variety of diverse cellular functions including growth control, proliferation, differentiation, immune response, tumor promotion, apoptosis and activation of viruses.

Forma física

Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide

Nota de preparación

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Alemania

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis
Lucie Aumailley et al.
PloS one, 10(10), e0140292-e0140292 (2015-10-09)
Werner syndrome (WS) is a premature aging disorder caused by mutations in a RecQ-family DNA helicase, WRN. Mice lacking part of the helicase domain of the WRN orthologue exhibit many phenotypic features of WS, including metabolic abnormalities and a shorter...
The curious case of peroxiredoxin-5: what its absence in aves can tell us and how it can be used
Pirson M, et al.
BMC Evolutionary Biology, 18(1), 18-18 (2018)
S Lapointe et al.
Biology of reproduction, 58(3), 747-753 (1998-03-25)
Hydrogen peroxide (H2O2) is a reactive oxygen species that at low concentration is toxic to sperm. H2O2 inhibits not only sperm viability but also the acrosome reaction, sperm-egg binding, and oocyte penetration. Catalase activates the decomposition of H2O2 into water...
J M Matés et al.
Frontiers in bioscience : a journal and virtual library, 4, D339-D345 (1999-03-17)
Aerobic organisms possess antioxidant defense systems that deal with reactive oxygen species (ROS) produced as a consequence of aerobic respiration. Reactive oxygen is related to both, the arrest of growth and the start of cell differentiation. Low concentrations of reactive...
E C Kaal et al.
Journal of neuroscience research, 54(6), 778-786 (1998-12-18)
Evidence is growing that reactive oxygen species (ROS), by-products of (normal) cellular aerobic metabolism, are involved in the pathogenesis of neurodegenerative diseases. One of these diseases is amyotrophic lateral sclerosis (ALS), in which motoneurons die, leading to paralysis and death....

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