C-12005

Normal Human Epidermal Keratinocytes (NHEK)

Juvenile foreskin, pooled, 500,000 cryopreserved cells

• Sinónimos: Human Keratinocyte Culture, NHEK Cell Line
• NACRES: NA.81
• UNSPSC Code: 41106514
• eCl@ss: 32160703
• Biological source: human foreskin (juvenile epidermis)
• Morphology: ( epidermal)

Propiedades

• biological source: human foreskin (juvenile epidermis)
• product line: PromoCell^®
• packaging: pkg of 500,000 cells
• morphology: ( epidermal)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

General description

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Application

Primary Normal Human Epidermal Keratinocytes (NHEK) are isolated from the epidermis of juvenile foreskin from pooled donors and are provided in a cryopreserved format. They are the major cell type in the epidermis, making up about 90% of the cells.Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

Biochem/physiol Actions

Skin

Preparation Note

Subculture Routine-Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Normal Human Epidermal Keratinocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, immunohistochemical tests for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 5000 cells per cm²Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable