As mentioned in the Preparation Note on the product webpage, the collagen is "prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.
The presence of Atelo or Telo collagen is not determined.
C7774
Human Collagen Type I
from human placenta, powder, suitable for cell culture
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Permítanos ayudarleNombre del producto
Colágeno from human placenta, Bornstein and Traub Type I (Sigma Type VIII), powder
biological source
human placenta
form
powder
technique(s)
cell culture | mammalian: suitable
impurities
HIV, hepatitis B and hepatitis C, none detected
storage temp.
2-8°C
Quality Level
Gene Information
bovine ... CSN2(281099), CSN3(281728)
human ... COL4A3(1285), COL4A4(1286), COL4A6(1288)
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1 of 4
Este artículo | C4407 | C5608 | C5483 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | stem cell: suitable |
| biological source human placenta | biological source human placenta | biological source rabbit skin | biological source human |
| form powder | form powder | form powder | form powder |
| Gene Information bovine ... CSN2(281099), CSN3(281728) | Gene Information human ... COL3A1(1300) | Gene Information rabbit ... COL1A2(100008997) | Gene Information human ... COL1A2(1278) |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| impurities HIV, hepatitis B and hepatitis C, none detected | impurities HIV, hepatitis B and hepatitis C, none detected | impurities - | impurities - |
Other Notes
El colágeno se clasifica en una serie de distintos tipos desde el punto de vista estructural y genético. Nosotros utilizamos la nomenclatura propuesta por Bornstein y Traub. Tenga cuidado de no confundir las designaciones de tipo Sigma con los tipos de clasificación de colágeno reconocidos
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.
Application
This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.
Biochem/physiol Actions
Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.
Preparation Note
Prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Instructions
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Is Human Collagen Type I, (Cat # C7774) isolated enzymatically or by acid extraction? Atelo or Telo?
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해당 제품의 순도가 궁금합니다.
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Unfortunately, the purity of this product is not determined. An SDS-PAGE is performed and the electrophoretic profile is confirmed to be consistent with past lots. For additional information please see the link below to the product datasheet:
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