C8218

Sigma-Aldrich

Monoclonal Anti-Cdk4 antibody produced in mouse

clone DCS-31, ascites fluid

Número MDL:
NACRES:
NA.41

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

ascites fluid

antibody product type

primary antibodies

clon

DCS-31, monoclonal

mol peso

antigen 33 kDa

contiene

15 mM sodium azide

species reactivity

human, rat, mouse

aplicaciones

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:1,000 using a cultured human tumor cell line extract

isotipo

IgG2a

conjugado

unconjugated

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... CDK4(1019)
mouse ... Cdk4(12567)
rat ... Cdk4(94201)

Descripción general

Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein.
Monoclonal Anti-Cdk4 (mouse IgG2a isotype) is derived from the DCS-31 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin-dependent kinase 4 is localized in human chromosome 12q14.1.

Especificidad

The antibody reacts specifically with Cdk4 and does not recognize other Cdk types.

Inmunógeno

recombinant human Cdk4 protein

Aplicación

Monoclonal Anti-Cdk4 antibody was used in:
  • immunoprecipitation of human melanoma cell line.
  • immunofluorescence of fibrosarcoma cells.
  • western blot analysis
  • immunofluorescence
  • immunohistochemistry

Acciones bioquímicas o fisiológicas

In association with cyclins, cyclin-dependent kinases (CDKs) forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Alemania

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Certificado de origen

Yan Liu et al.
BMC cell biology, 10, 1-1 (2009-01-13)
Regenerative wound repair is a goal of modern medicine. This is important not only for the local repair but also for its beneficial effect to systemic physiological processes. When wounds become chronic, individuals are susceptible to generalized inflammatory cascades that...
Grazia Saturno et al.
Toxicologic pathology, 35(7), 972-983 (2007-12-22)
Protein-kinase inhibitors are among the most advanced compounds in development using the new drug discovery paradigm of developing small-molecule drugs against specific molecular targets in cancer. After treatment with a cyclin dependent kinase CDK2 inhibitor in monkey, histopathological analysis of...
Adam Z Oskowitz et al.
The international journal of biochemistry & cell biology, 43(11), 1563-1572 (2011-07-29)
Recently we demonstrated that the miRNA regulate human mesenchymal stem cells (hMSCs) differentiation. To determine the role of the miRNA pathway in hMSCs proliferation, Drosha and Dicer knockdown hMSCs were generated using a lentiviral based tetracycline inducible shRNA. hMSCs with...
Yamashita, M., et al.
Development, Growth & Differentiation, 33, 617-617 (1991)
J Pines et al.
The Journal of cell biology, 115(1), 1-17 (1991-10-01)
We have used immunofluorescence staining to study the subcellular distribution of cyclin A and B1 during the somatic cell cycle. In both primary human fibroblasts and in epithelial tumor cells, we find that cyclin A is predominantly nuclear from S...
Artículos
Quantitative and qualitative western blotting to validate knockdown by esiRNA.
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