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https://www.sigmaaldrich.com/product/sigma/c9268#product-documentation
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/422/794/c9268enz.pdf
C9268
Carboxypeptidase A from bovine pancreas
(Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution
Sinónimos:
Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
500 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 119,00 € |
2500 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 402,00 € |
5000 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 436,00 € |
Acerca de este artículo
Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
Número CE:
MDL number:
Specific activity:
≥50 units/mg protein
119,00 €
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Servicio técnico
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Proteomics Grade
Quality Segment
form
ready-to-use solution
quality
(Type II-PMSF treated)
specific activity
≥50 units/mg protein
mol wt
~35 kDa
purified by
2× crystallization
impurities
≤0.05 BTEE units/mg protein chymotrypsin, ≤10 BAEE units/mg protein trypsin
storage temp.
2-8°C
Application
The enzyme from Sigma has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose.
Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.
Biochem/physiol Actions
Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.
Preparation Note
Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added.
Analysis Note
Protein determined by E1%/278
Other Notes
One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.
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Este artículo | |||
|---|---|---|---|
| description (Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution | description ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas | description TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein | description (Type II-PMSF treated), ≥20 units/mg protein |
| specific activity ≥50 units/mg protein | specific activity ≥6 units/mL packed gel, 25 °C | specific activity ≥10,000 BAEE units/mg protein | specific activity ≥20 units/mg protein |
| grade Proteomics Grade | grade - | grade Proteomics Grade | grade - |
| form ready-to-use solution | form ammonium sulfate suspension | form essentially salt-free, lyophilized powder | form aqueous suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt ~35 kDa | mol wt ~35,250 | mol wt 23.8 kDa | mol wt ~35 kDa |
| impurities ≤10 BAEE units/mg protein trypsin, ≤0.05 BTEE units/mg protein chymotrypsin | impurities - | impurities - | impurities ≤10 BAEE units/mg protein trypsin, ≤0.05 BTEE units/mg protein chymotrpsin |
Palabra de advertencia
Danger
Códigos de peligro
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
10 - Combustible liquids
¿Qué está pasando?
WGK 2
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Listados normativos
Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.
108-88-3
CAS No.
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