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D0426
DAB with Metal Enhancer
SIGMAFAST™, peroxidase substrate, tablet
Sinónimos:
3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate
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UNSPSC Code:
12352204
NACRES:
NA.83
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Permítanos ayudarleNombre del producto
SIGMAFAST™ DAB with Metal Enhancer, tablet
form
tablet
solubility
water: 1 tablet/5 mL
storage temp.
−20°C
Quality Level
Categorías relacionadas
1 of 4
Este artículo | D4418 | P9187 | D4293 |
|---|---|---|---|
| solubility water: 1 tablet/5 mL | solubility water: 1 tablet/5 mL | solubility water: 0.5 tablet/10 mL, clear, colorless | solubility - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form tablet | form tablet | form tablet | form tablet |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Application
SIGMAFAST™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry,[1] dorsal root ganglion (DRG) neurons[2] and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.[3]
The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.
General description
SIGMAFAST™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.
Preparation Note
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.
signalword
Danger
Hazard Classifications
Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
Clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
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Somanshu Banerjee et al.
Journal of photochemistry and photobiology. B, Biology, 176, 124-135 (2017-10-11)
In most of the avian species, daylength or photoperiod is the main environmental factor regulating reproduction. During their annual gonadal cycle, birds once sensitive to short or long day effect develop refractoriness to the same daylength and gonad develop or
Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture
Cheng M, et al.
Tissue Engineering: Part A, 16(5), 1479-1489 (2010)
Developing rat lung has a sided pacemaker region for morphogenesis-related airway peristalsis
Jesudason EC, et al.
American Journal of Respiratory Cell and Molecular Biology, 32(2), 118-127 (2005)
Cdk5 inhibitor roscovitine alleviates neuropathic pain in the dorsal root ganglia by downregulating N-methyl-D-aspartate receptor subunit 2A
Yang L, et al.
Neurological Sciences, 35(9), 1365-1371 (2014)
Germain J P Fernando et al.
Journal of controlled release : official journal of the Controlled Release Society, 237, 35-41 (2016-07-07)
DNA vaccines have many advantages such as thermostability and the ease and rapidity of manufacture; for example, in an influenza pandemic situation where rapid production of vaccine is essential. However, immunogenicity of DNA vaccines was shown to be poor in
Artículos
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Protocolos
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
Contenido relacionado
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What is the Department of Transportation shipping information for this product?
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What is a tablet set for Product D0426, SIGMAFAST™ DAB with Metal Enhancer?
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This product contains two substrate tablets per tablet set; there is one DAB/cobalt tablet and one urea/hydrogen peroxide tablet. Both tablets must be used in the assay.
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What is the metal enhancer in Product D0426, SIGMAFAST™ DAB with Metal Enhancer, and what is the purpose of it?
1 respuesta-
The metal enhancer is cobalt chloride (CoCl2). Cobalt chloride produces a more intense stain which enhances the sensitivity of the assay and allows for visualization of the staining when a standard DAB chromogen would fail to demonstrate the staining.
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How can I block endogenous peroxidase activity when using Product D0426, SIGMAFAST™ DAB with Metal Enhancer, in immunohistochemistry?
1 respuesta-
Endogenous peroxidase activity may be blocked by flooding the slide with a solution of 4 parts methanol and 1 part 3% hydrogen peroxide.
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