D1667

Sigma-Aldrich

Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse

clone 440.4, ascites fluid

Número MDL:
NACRES:
NA.41

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

ascites fluid

antibody product type

primary antibodies

clon

440.4, monoclonal

mol peso

antigen 440 kDa

contiene

15 mM sodium azide

species reactivity

human, chicken

aplicaciones

indirect ELISA: suitable
western blot: 1:100 using a chick brain extract or Kinesin enriched rat brain extract

isotipo

IgG2a

conjugado

unconjugated

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... DNAH1(25981), DNAH2(146754), DNAH3(55567), DNAH5(1767)

Descripción general

Monoclonal Anti-Dynein (Heavy Chain) (mouse IgG2a isotype) is derived from the 440.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Dynein axonemal heavy chain 1 (DNAH1) encodes for an inner dynein heavy chain. This gene is mapped to the human chromosome location 3p21.1. DNAH1 is expressed in the testis and other ciliated cells. Dyneins are composed of two or three distinct heavy chains, three intermediate chains and at least four light chains.

Inmunógeno

cytoplasmic dynein from chicken embryo brain.

Aplicación

Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse has been used in:
  • indirect immunofluorescence at 1:50 dilution
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • dot blot

Acciones bioquímicas o fisiológicas

Dynein is a motor protein that regulates the movement of organelles to the ‘minus′ end of microtubules. These motor proteins are also involved in spindle organization, retrograde transport and chromosome movement
Dynein has also been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport. It also regulates the endocytic pathway and the organization of the Golgi apparatus. Mutations in dynein axonemal heavy chain 1 (DNAH1) are associated with primary ciliary dyskinesia and multiple morphological anomalies of the flagella (MMAF) that leads to asthenozoospermia and male infertility.

Forma física

Suppied as ascites fluid with 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Mariem Ben Khelifa et al.
American journal of human genetics, 94(1), 95-104 (2013-12-24)
Ten to fifteen percent of couples are confronted with infertility and a male factor is involved in approximately half the cases. A genetic etiology is likely in most cases yet only few genes have been formally correlated with male infertility....
L Wordeman et al.
The Journal of cell biology, 114(2), 285-294 (1991-07-01)
We have used indirect immunofluorescence in combination with correlative EM to subdivide the mammalian kinetochore into two domains based on the localization of specific antigens. We demonstrate here that the fibrous corona on the distal face of the kinetochore plate...
R E Fowler et al.
Molecular and biochemical parasitology, 117(2), 187-200 (2001-10-19)
We have studied the occurrence, stage specificity and cellular location of key molecules associated with microtubules in Plasmodium falciparum merozoites. Antibodies to gamma tubulin, conventional kinesin and cytoplasmic dynein were used to determine the polarity of merozoite microtubules (mt), the...
Jason Yi et al.
The Journal of cell biology, 202(5), 779-792 (2013-08-28)
T cells rapidly reposition their centrosome to the center of the immunological synapse (IS) to drive polarized secretion in the direction of the bound target cell. Using an optical trap for spatial and temporal control over target presentation, we show...
X Wang et al.
Clinical genetics, 91(2), 313-321 (2016-08-31)
This study aimed to investigate the genetic pathogeny of multiple morphological anomalies of the flagella (MMAF), which is a genetically heterogeneous disorder leading to male infertility. Nine patients with severe asthenozoospermia caused by MMAF were recruited. Whole genome sequencing and...

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