This DMSO product has not been tested for suitability in HPLC applications. Please see the links below to review DMSO products suitable for HPLC:
https://www.sigmaaldrich.com/product/sigald/34869
https://www.sigmaaldrich.com/product/saj/074871
https://www.sigmaaldrich.com/product/sial/94563
D2650
Dimetilsulfóxido
Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Dimethyl sulfoxide
Sinónimos:
DMSO
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Fórmula lineal:
(CH3)2SO
Número CAS:
Peso molecular:
78.13
Beilstein:
506008
Número CE:
Número MDL:
Código UNSPSC:
12191502
ID de la sustancia en PubChem:
NACRES:
NA.75
Bp:
189 °C (lit.)
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Servicio técnico
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Hybri-Max™
Nivel de calidad
densidad de vapor
2.7 (vs air)
presión de vapor
0.42 mmHg ( 20 °C)
esterilidad
sterile-filtered
Línea del producto
BioReagent
Ensayo
≥99.7%
Formulario
liquid
temp. de autoignición
573 °F
lim. expl.
42 %, 63 °F
técnicas
cell culture | hybridoma: suitable
cryopreservation: suitable
impurezas
endotoxin, tested
≤0.1% water
water
índice de refracción
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubilidad
H2O: miscible (completely)
densidad
1.10 g/mL (lit.)
cadena SMILES
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
Clave InChI
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
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Este artículo | D2653 | D2438 | D4540 |
|---|---|---|---|
| technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) cell culture | plant: suitable, cryopreservation: suitable |
| Quality Level 400 | Quality Level 300 | Quality Level 400 | Quality Level 200 |
| grade Hybri-Max™ | grade Hybri-Max™ | grade BioPerformance Certified | grade - |
| assay ≥99.7% | assay ≥99.7% | assay - | assay ≥99.5% (GC) |
| solubility H2O: miscible (completely) | solubility H2O: miscible (completely) | solubility H2O: miscible (completely) | solubility H2O: miscible (completely) |
| form liquid | form liquid | form liquid | form liquid |
Aplicación
El DMSO es un disolvente aprótico polar utilizado en reacciones químicas, en reacciones en cadena de la polimerasa (PCR) y como agente de vitrificación crioprotector para la conservación de células, tejidos y órganos. El DMSO se utiliza en los medios de congelación celular para proteger a las células de las lesiones mecánicas inducidas por el cristal de hielo. Se utiliza para el almacenamiento en congelación de líneas celulares de hibridomas y heteroploides primarias, subcultivadas y recombinantes; células madre embrionarias (ESC) y células madre hematopoyéticas. El DMSO se utiliza con frecuencia en combinación con la seroalbúmina bovina (BSA) o el suero bovino fetal (FBS).
Envase
100 ml en botella de vidrio
El embalaje de ampollas de vidrio está ahora disponible bajo un nuevo número de referencia, D2653
Otras notas
Este producto es un producto Hybri-Max. Se analiza en hibridomas y se evalúa su idoneidad para la congelación celular. Este producto está esterilizado mediante filtración (0,2 micras) y probado para determinar la concentración de endotoxinas.
Información legal
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Cláusula de descargo de responsabilidad
Fácilmente superenfriado, vuelve a fundir lentamente a temperatura ambiente. Producto solidificado que puede volver a licuarse por calentamiento a temperatura ambiente sin detrimento del producto.
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
188.6 °F - closed cup
Punto de inflamabilidad (°C)
87 °C - closed cup
Equipo de protección personal
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Artículos
Overview of good cell banking practices for cell line cryopreservation purposes.
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Protocolos
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.
Human ips cell passaging protocol using EZ-LiFT™ Reagent, an enzyme-free and chemically defined pluripotent stem cell dissociation reagent.
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Contenido relacionado
Product Information Sheet
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Is this DMSO suitable for HPLC?
1 respuesta-
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Is this DMSO suitable to be used as a drug solvent in animal studies?
1 respuesta-
This product has not been tested for use in animal models. However, this is a sterile-filtered DMSO that has been tested for cytotoxicity and is expected to be suitable for in vivo research applications.
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How many days after opening the bottle is DMSO safe to use?
1 respuesta-
This product is assigned an expiration date of approximately two years from the date of Quality Release. This date is lot specific and available on the product label and Certificate of Analysis. After opening, the material should be stored tightly sealed to prevent moisture uptake. Given proper handling and storage, the product should be stable until the published expiration date.
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I ordered 10 x 10ml vials for fibroblast culture work. My first endeavor in culture domain. What are the storage conditions for DMSO of this grade? This arrived in plain cardboard box at room temperature.
1 respuesta-
DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots with inert gas and store them in a compatible, tightly sealed container, at a cool storage area, such as 4°C. DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Safety Data Sheet and the Product Information Sheet for compatibility of DMSO with various materials and for further details.
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Is it safe in terms of health and product safety to store a leftover DMSO ampule covered with parafilm in a flammable cabinet?
1 respuesta-
DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots and store them under inert gas, in a compatible, tightly sealed container, at a cool storage area, such as 4°C.
DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Product Information Sheet and the Safety Data Sheet at the link below for compatibility of DMSO with various materials and for further details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265
¿Le ha resultado útil?
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Is this anhydrous?
1 respuesta-
This product is not considered anhydrous. The maximum allowable water content is 0.1% which is reported on the lot-specific Certificate of Analysis. Please see the link below to review the anhydrous DMSO products available:
https://www.sigmaaldrich.com/search/dmso-anhydrous?focus=products&page=1&perpage=30&sort=relevance&term=DMSO%20anhydrous&type=product¿Le ha resultado útil?
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I ordered 10 ml glass vials, used 7 ml, Can I store it in centrifuge tubes? For how long can I use that?
1 respuesta-
It may be best to store DMSO in amber glass vials for long term storage, as this compound is not compatible with many types of plastics. The compatibility of DMSO with various materials is listed below:
Compatible: LDPE, HDPE, polypropylene, PPCO(polypropylene copolymer), polymethylpentene, nylon, teflon FEP
Moderately compatible: polystyrene, ECTFE/ETFE
Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonateNote that DMSO is hygroscopic and light sensitive. The storage container selected must offer protection from light and moisture uptake. The product is assigned a firm expiration date. Please refer to the lot specific Certificate of Analysis to assume the material is within the expected shelf life.
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E' solo ad uso per ricerca o utilizzabile anche in diagnostica/human? Grazie
1 respuesta-
This product is not suitable for diagnostic/human use.
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Is this product packaged in a bottle with a septa cap?
1 respuesta-
No, this product is not packaged with a septa cap. It comes in a 125 ml amber glass bottle with a 28 mm solid white cap.
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Can I use it for PCR?
1 respuesta-
While this product is tested specifically for use in cell culture, there is no reason to suspect that it would not perform well in PCR applications. The product is NOT tested for amplification, DNAse, or nuclease activity. Should this testing be important, see the link below to product options that include this testing:
D9170 DMSO PCR Reagent:
https://www.sigmaaldrich.com/product/sigma/d9170
D8418 DMSO Molecular Biology Tested
https://www.sigmaaldrich.com/US/en/product/sigma/d8418¿Le ha resultado útil?
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