Looking on the datasheet, it states that there is 250 ug of DNA per vial and that the vial has 100 x 5uL loads (500 uL volume). DNA concentration is 0.5 ug/uL. Loading 5 uL per well therefore provides a DNA load of 2.5 ug per well. Please view the data sheet for more information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/157/148/d3937dat.pdf
D3937
DirectLoad™ 1 kb DNA Ladder
ready-to-use marker for DNA electrophoresis
Sinónimos:
1 kb marker, 1kb gel marker, DNA marker, agarose gel electrophoresis marker
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 vial | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 256,00 € |
Acerca de este artículo
NACRES:
NA.25
UNSPSC Code:
41105335
eCl@ss:
32119000
256,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleQuality Segment
form
liquid
usage
100 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s DirectLoad™ 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.
Application
Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Other Notes
Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Sigma′s DirectLoad™ 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
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Este artículo | |||
|---|---|---|---|
| description ready-to-use marker for DNA electrophoresis | description for DNA electrophoresis | description ready-to-use marker for DNA electrophoresis | description ready-to-use marker for DNA electrophoresis |
| form liquid | form liquid | form (liquid) | form liquid |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
| usage 100 uses | usage - | usage sufficient for 100 loads | usage 75 uses |
ladder | Marker, Ladder, NA electrophoresis | NA electrophoresis, marker, ladder | - |
Clase de almacenamiento
12 - Non Combustible Liquids
¿Qué está pasando?
WGK 2
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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