See the link below to review the formulations of the DPBS products. These concentrations are reported in grams per liter:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/269/385/d5652for.pdf
D5652
Disolución salina tamponada con fosfatos de Dulbecco
Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture
Sinónimos:
DPBS
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Servicio técnico
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Permítanos ayudarleQuality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
pH
7.2-7.8
solubility
water: 9.6 g/L, clear
storage temp.
2-8°C
General description
Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.
Application
Dulbecco′s Phosphate Buffered Saline has been used:
- as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy
- to dissolve Angelica gigas powder for microencapsulation
- to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay
- to wash amniotic membrane for cellular analyses
D-PBS is used to wash cells during preparation and serial transfer.
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
Other Notes
This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available
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Este artículo | |||
|---|---|---|---|
| description Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture | description With calcium chloride and magnesium chloride, 10×, liquid, sterile-filtered, suitable for cell culture | description With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | description Modified, with 36 mg sodium pyruvate, 50 mg streptomycin sulfate, 100 mg kanamycin monosulfate, 1000 mg glucose/L and CaCl2, liquid, 0.1 μm filtered, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 500 | Quality Level 400 | Quality Level 400 | Quality Level 200 |
| form powder | form liquid | form liquid | form liquid |
| solubility water: 9.6 g/L, clear | solubility - | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C | storage temp. 2-8°C |
| pH 7.2-7.8 | pH - | pH - | pH - |
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the formulation for this buffer? Molarity of Na2HPO4, KH2PO4, NaCl, KCl, etc?
1 respuesta-
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What is the pH of Product D5652, Dulbecco's Phosphate Buffered Saline?
1 respuesta-
The specification for the pH of D5652, Dulbecco's Phosphate Buffered Saline (when dissolved in water) is 7.2 to 7.8.
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What is the sodium chloride concentration in Product D5652, Dulbecco's Phosphate Buffered Saline?
1 respuesta-
This product contains 8.0 g/L sodium chloride.
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Can Product D5652, Dulbecco's Phosphate Buffered Saline, be autoclaved?
1 respuesta-
Product No. D5652, Dulbecco's Phosphate Buffered Saline should be autoclaved or sterile-filtered before use. It can be autoclaved at 121°C, 15 psi for 20 minutes.
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Is Product D5652, Dulbecco's Phosphate Buffered Saline, offered as a powder or a liquid?
1 respuesta-
Product No. D5652, Dulbecco's Phosphate Buffered Saline, is a powder.
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Is Product D5652, Dulbecco's Phosphate Buffered Saline, a 1X solution once it has been diluted?
1 respuesta-
Product No. D5652, DPBS is a 1X solution after reconstitution at 9.6 g/L.
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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