This product contains 3.7 g/L of NaHCO3.
D5796
DMEM - high glucose
With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
Sinónimos:
DME, DMEM
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UNSPSC Code:
12352207
NACRES:
NA.75
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Servicio técnico
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Permítanos ayudarleNombre del producto
Medio de Eagle modificado de Dulbecco, glucosa elevada, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Este artículo | D5671 | D6429 | D6546 |
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form liquid | form liquid |
| components NaHCO3: yes | components L-glutamine: no | components HEPES: no | components NaHCO3: yes |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
Application
El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».
General description
Este medio de glucosa DMEM-Hi es un medio completo 1x sin factores añadidos (modificaciones comunes) como L-alanil-L-glutamina, HEPES o piruvato sódico. Difiere de la formulación DMEM-Hi original en que se sustituye la piridoxina por el piridoxal. El piridoxal es un componente inestable de los medios.
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Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
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What is the NaHCO3 concentration? Conventional cell culture requires 5% of CO2. But it will depend on the NaHCO3 concentration. Does this product use 1.2 or 3.7 g/L?
1 respuesta-
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What are the product numbers for DMEM and EMEM used to grow the 293 and 293T cell lines?
1 respuesta-
To grow the 293 and 293T cells, the following product recommendations are provided:
For the 293 cell line (product number 85120602), ECACC recommends using EMEM media (EBSS) with 2mM Glutamine, 1% Non Essential Amino Acids (NEAA), and 10% FCS. ECACC uses the product number M4655 for this purpose.
For the 293T cell line (product number 12022001), ECACC recommends using DMEM media with 2mM Glutamine and 10% Fetal Bovine Serum (FBS). ECACC uses the product number D5796 media for sub-culturing this cell line.
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What is the conc. of L glutamine
1 respuesta-
The concentration of L-Glutamine in this product is 0.584 g/L. Please see the link below to review the DMEM Formulation Tables: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/340/109/d5796for.pdf
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pH는 몇으로 조정되어 있나요?
1 respuesta-
The pH specification of this product is 7.0-7.6. The exact value will be reported in the lot specific Certificate of Analysis. Please see the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/coa/SIGMA/D5796/RNBL9771.
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