This product can be reconstituted in water at 01. mg/mL. The solubility in PBS has not been determined, but is expected to be similar. Please see the link below to review additional preparation instructions included in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/272/751/d8935dat.pdf
D8935
Nidogen-1 (NID1) human
recombinant, expressed in HEK 293 cells, suitable for cell culture
Sinónimos:
Entactin, NID, NID-1, NID1
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
50 μg | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 296,00 € |
Acerca de este artículo
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
95% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells
296,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlerecombinant
expressed in HEK 293 cells
assay
95% (SDS-PAGE)
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
storage temp.
−20°C
General description
Nidogen-1 belongs to the nidogens family of glycoproteins. This family consists of nidogen-1 and nidogen-2, both of which are sulfated monomeric glycoproteins. Nidogen-1 is ubiquitously present in the synaptic and extra synaptic basal lamina. It is mapped to human chromosome 1q42.3. Nidogen-1 encodes a 139 kDa, three globular domains namely G1, G2 and G3 and has multiple epidermal growth factor repeats. Recombinant human nidogen-1 is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 133.5 kDa (amino acids 29-1247). This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
Application
This recombinant human nidogen-1 product can be used as an attachment factor for cells adherence.
Biochem/physiol Actions
Nidogen-1 (NID-1) is thought to have an important role in the construction of basement membrane by linking between the networks of laminin and collagen IV. Genetic elimination of the nidogen-1 binding site on the mouse laminin γ1 chain leads to impaired deposition of basement membranes and hampers microvessel maturation. Nidogen 1 and 2 double knockout in mouse fibroblasts led to the loss of all major basal membrane components from the epidermal-collagen interface, and complete abolishment of hemidesmosomes. Supplementation by either recombinant nidogen-1 or -2 improved basal membrane formation. Mutations in NID1and laminin γ1 is implicated in pathogenesis of Dandy-Walker spectrum disorders.
1 of 1
Este artículo | |||
|---|---|---|---|
| description recombinant, expressed in HEK 293 cells, suitable for cell culture | description from mouse(High Concentration), pack of 10.5 mg | description 1 mg/mL, solution, BioReagent, suitable for cell culture | description for serum, plasma, cell culture supernatants and urine |
| assay 95% (SDS-PAGE) | assay - | assay 95% (Human collagen type IV, SDS-PAGE) | assay - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell attachment: suitable, cell differentiation: suitable | technique(s) cell culture | mammalian: suitable | technique(s) ELISA: suitable, capture ELISA: suitable |
| recombinant expressed in HEK 293 cells | recombinant - | recombinant - | recombinant - |
| form lyophilized powder | form liquid | form solution, liquid | form - |
| storage temp. −20°C | storage temp. - | storage temp. −20°C | storage temp. −20°C |
Clase de almacenamiento
13 - Non Combustible Solids
WGK
WGK 1
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
-
What is the solubility of this protein in water and PBS?
1 respuesta-
¿Le ha resultado útil?
-