Merck
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E6758

Sigma-Aldrich

Ethylenediaminetetraacetic acid

anhydrous, crystalline, BioReagent, suitable for cell culture

Sinónimos:
Edathamil, (Ethylenedinitrilo)tetraacetic acid, EDTA, Ethylenedinitrilotetraacetic acid
Fórmula lineal:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
Número de CAS:
Peso molecular:
292.24
Beilstein:
1716295
Número de EC:
Número MDL:
eCl@ss:
39030907
ID de la sustancia en PubChem:
NACRES:
NA.25

grado

anhydrous

línea de producto

BioReagent

ensayo

≥98.5%

formulario

crystalline

technique(s)

cell culture | mammalian: suitable

mp

250 °C (dec.) (lit.)

solubilidad

3  M NaOH: 100 mg/mL

densidad

1.46 g/cm3 at 20 °C

trazas de catión

C: 40.7-41.5%
N: 9.3-9.8%

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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Aplicación

Ethylenediaminetetraacetic acid has been used:
  • as a component of peptide dilution buffer for intracellular BH3 (Bcl-2 homology (BH) domain) profiling and validation
  • as a medium component in the hepatic stellate cell (HSCs) culture
  • as a component of homogenization buffer for protein extraction from eye tissues
  • for stopping benzonase reaction in lysis buffer

Envase

100, 500 g in poly bottle

Acciones bioquímicas o fisiológicas

Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, majorly used in preventing clotting of blood samples. EDTA may trigger aggregation of platelets resulting in pseudothrombocytopenia. EDTA along with tetracycline imparts serves as root conditioning agent and provides relief in gum infection. EDTA-tetracycline therapy decreases coronary artery calcium levels in atherosclerosis. Calcium Disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Eye Irrit. 2

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificado de Análisis

Introduzca el número de lote para buscar un certificado de análisis (COA).

Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Lechuga A, et al.
Bio-protocol, 8(1), 1571-1571 (2018)
Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
Dai J and Luftig MA
Journal of Immunology, ji1701473-ji1701473 (2018)
The blockade of lipophagy pathway is necessary for docosahexaenoic acid to regulate lipid droplet turnover in hepatic stellate cells
Qiu S, et al.
Biomedicine and Pharmacotherapy, 109, 1841-1850 (2019)
Protein Expressions of the Small GTPase Rho Proteins in Pterygial Tissue and Leukocytes of Patients with Pterygium
Saracaloglu A, et al.
Proceedings, 2(25), 1571-1571 (2018)
Does calcium disodium EDTA slow CKD progression?
Weaver VM, et al.
American Journal of Kidney Diseases, 60(4), 503-506 (2012)

Protocolos

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

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