The molecular weight of this enzyme has not been analyzed. The enzymatic assay states a solubility of 1 mg/ ml in water.
Immediately prior to pipetting into cuvette, prepare a solution at 1 mg / mL in cold purified water. Place on ice.
This solution is stable for a maximum of thirty minutes.
F0628
Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach)
lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase
Oferta disponible
Sinónimos:
Ferridoxin-NADP+ oxidoreductase
Iniciar sesión para ver los precios por organización y contrato.
Gastar 450 € — Ahorrar 5 %; Gastar 700 € — Ahorrar 10 %; Gastar 1300 € — Ahorrar 15 %*
Iniciar sesión para comprobar la elegibilidad. Comprar productos subvencionables
Seleccione un Tamaño
Cambiar Vistas
| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 unit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 210,00 € |
10 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 751,00 € |
5 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 770,00 € |
Acerca de este artículo
Número CAS:
Número CE:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥15 units/mg solid
210,00 €
Ahorre hasta 15 %
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleform
lyophilized powder
Quality Segment
specific activity
≥15 units/mg solid
foreign activity
NADH diaphorase activity ≤10 units/mg solid
storage temp.
−20°C
General description
Ferredoxin-NADP+ Reductase is a ferredoxin-soluble partner with a molar ratio to PSI of 0.9 and 3.
Application
Ferredoxin-NADP+ Reductase was used in in vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes. It was also used to regulate glyceraldehyde-3-phosphate dehydrogenase.
Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach) has been used to test the effect of potential protein partners on the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)−CP12 preparation. It has also been used in standard assay conditions for phycocyanobilin:ferredoxin oxidoreductase (PcyA) enzyme assay.
Biochem/physiol Actions
Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.
Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.
Other Notes
One unit will reduce 1.0 millimole of cytochrome C per min at pH 7.5 at 25 °C in the presence of spinach ferredoxin and NADP.
1 of 1
Este artículo | |||
|---|---|---|---|
| description lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase | description lyophilized powder, Ferredoxin ≥15 wt. % | description buffered aqueous solution | description partially purified powder, 0.01-0.1 unit/mg solid |
| specific activity ≥15 units/mg solid | specific activity - | specific activity - | specific activity 0.01-0.1 unit/mg solid |
| form lyophilized powder | form lyophilized powder | form buffered aqueous solution | form partially purified powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| foreign activity NADH diaphorase activity ≤10 units/mg solid | foreign activity - | foreign activity - | foreign activity - |
Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
-
Hi, how can I prepare a stock solution with the 1 U vial? It's not very clear for me in the specification sheet. I need a 1 uM final concentration of reductase for my assay, how do I prepare a stock solution of it?
1 respuesta-
¿Le ha resultado útil?
-
-
Hi there, could you please provide the specific methodology to extract this lyophilized powder from the vial? I didn't know how to extract this ferredoxin-NADP+ reductase from the vial
1 respuesta-
The vials contain less than 1mg solid so reconstituting the whole vial is recommended.
¿Le ha resultado útil?
-