This product may be stored at room temperature. Stability studies on the product in solution have not been completed. However, the literature suggests that solutions are fairly stable. See the link below to a publication citing solution stability:
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/calcofluor-white.
F3543
Fluorescent Brightener 28
used as a stain and brightening agent
Sinónimos:
Calcofluor White M2R, Tinopal UNPA-GX
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Fórmula empírica (notación de Hill):
C40H44N12O10S2
Número CAS:
Peso molecular:
916.98
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
224-548-7
MDL number:
Para consultas sobre este producto F3543, póngase en contacto con su oficina o distribuidor locales de Merck. Póngase en contacto con el Servicio técnico
Servicio técnico
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Permítanos ayudarleform
powder
mp
300 °C
εmax
40-60 at 238-242 nm in water at 0.01 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
OCCN(CCO)c1nc(Nc2ccccc2)nc(Nc3ccc(\C=C\c4ccc(Nc5nc(Nc6ccccc6)nc(n5)N(CCO)CCO)cc4S(O)(=O)=O)c(c3)S(O)(=O)=O)n1
InChI
1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+
InChI key
CNGYZEMWVAWWOB-VAWYXSNFSA-N
General description
Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps. It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.
Fluorescent Brightener 28 has been used for the staining of fungal cell walls. It has also been used for the staining of Candida albicans biofilm.
Application
It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.
Palabra de advertencia
Warning
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Eye Irrit. 2
Clase de almacenamiento
13 - Non Combustible Solids
¿Qué está pasando?
WGK 1
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
dust mask type N95 (US), Eyeshields, Gloves
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What is the storage temperature of fluorescent brightener 28?
1 respuesta-
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What are the excitation and emission wavelengths for Product F3543, Fluorescent Brightner 28?
1 respuesta-
Excitation is at 365 nm and emission at is at 435 nm.
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How is Product F3543, Fluorescent Brightner 28, dissolved for use?
1 respuesta-
The product can be diluted in DMSO at a concentration of 50 mg/ml. The product can be dissolved in water at a concentration of 1 mg/ml, although the use of 50% sodium hydroxide might be required to fully solubilize the product.
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Is there a reference for use of Product F3543, Fluorescent Brightner 28?
1 respuesta-
3 different staining applications are found in the following reference: Clark, G; Staining Procedures, 4th Edition, Williams & Wilkins, Baltimore 1981 pages 46-48. This product is used to monitor removal or regeneration of plant cell walls. A protocol for using this product with plant protoplasts is described. D.W. Galbraith, Physiologia Plantarum, 53, 111-116 (1981).
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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