No. F4680 is not recommended for mounting Oil Red O stained slides. Mounting medias suitable for use with Oil Red O are glycerol gelatin, Kaiser's Glycerol Gelatin(e) and glycerol or glycerol diluted with PBS.
There is a glycerol gelatin available that is suitable for mounting Oil Red O slides. The catalog number is GG1. To use either glycerol gelatin or Kaiser's glycerol gelatin(e) the bottle must be warmed to approximately 60°C before use. The mounting media is sold at room temperature and returns to a solid at approximately 37°C
F4680
Medio de montaje acuoso Fluoromount™
liquid
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
25 mL | Disponible para enviar HOYdesdeMerck Life Science S.A.S | 142,00 € |
Acerca de este artículo
UNSPSC Code:
12171500
NACRES:
NA.47
Servicio técnico
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Permítanos ayudarleNombre del producto
Medio de montaje acuoso Fluoromount™, for use with fluorescent dye-stained tissues
Quality Segment
form
liquid
technique(s)
microbe id | staining: suitable
color
clear
suitability
suitable for fluorescence, suitable for immunohistochemistry
application(s)
diagnostic assay manufacturing
hematology
histology
General description
Fluoromount es un medio de montaje acuoso, similar al Gel Mountä, para utilizar con cubreobjetos para preservar los tejidos teñidos con tintes fluorescentes. Este producto es compatible con el marcaje FITC, ficoeritrina, ficocianina y aloficocianina. También puede utilizarse con otros marcadores fluorescentes como TexasRedÒ y rodamina. Después de su uso el medio puede quitarse fácilmente dejando en remojo los cubreobjetos en agua desionizada.
Application
Legal Information
Fluoromount is a trademark of Diagnostic BioSystems, Inc.
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Este artículo | |||
|---|---|---|---|
| description for use with fluorescent dye-stained tissues | description tissue mounting medium | description This Fluorescent Mounting Media, Aqueous is validated for use in Immunofluorescence for the detection of Fluorescent Mounting Media Aqueous. | description - |
| technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - | technique(s) - |
| suitability suitable for immunohistochemistry, suitable for fluorescence | suitability - | suitability - | suitability - |
| color clear | color - | color - | color - |
| application(s) diagnostic assay manufacturing | application(s) hematology | application(s) - | application(s) hematology |
| form liquid | form liquid | form liquid | form - |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 100 |
Clase de almacenamiento
10 - Combustible liquids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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Can this product be used for mounting Oil Red O stained slides?
1 respuesta-
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Can you explain why it's recommended not to refrigerate it? Does that still apply even after opening?
1 respuesta-
This product is provided as a clear, viscous solution. Refrigeration may cause it to become difficult to use for coverslipping. Its product Information states, "Do not refrigerate". This applies to storage after the product has been opened.
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What are some examples of aqueous mounting media without glycerol?
1 respuesta-
For use with fluorescence:
1. F4680 Fluoromount
2. F6182 FluoroshieldAqueous Mounting media with no glycerol and antifade agents:
1. 10981 Polyvinyl alcohol with DABCO
2. F6937 Fluoroshield with DABCO
3. F6812 Fluoroshield with PPD (not suitable for a select number of fluorescent dyes)Aqueous Mounting Media with no glycerol and another dye:
1. F6057 Fluoroshield with DAPI
2. F5932 Fluoroshield with PIAqueous Mounting Media suitable for use with light microscopy:
1.GG1 Glycerol gelatin
2.C9368 CC/Mount¿Le ha resultado útil?
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Is F4680 suitable for use as a mounting medium for AEC red IHC mounting?
1 respuesta-
Fluoromount is not intended for use with bright field microscopy or for coverslipping slides stained with AEC. Most fluorescent mounting media are somewhat alkaline, with a pH around 9, while AEC is typically formulated with an acetate buffer around pH 5.2. The difference in pH levels suggests that the AEC may fade quickly, especially if the slides are counterstained with a nuclear counterstain such as Mayer's Hematoxylin, which would also likely disappear rapidly.
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Does this (Fluoromount F4680) contain DAPI?
1 respuesta-
This product does not contain DAPI. Should DAPI be preferred, see product F6057.
F4680 Fluoromount datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/692/371/f4680pis.pdfF6057 Fluoroshield with DAPI product page:
https://www.sigmaaldrich.com/product/sigma/f6057¿Le ha resultado útil?
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Does Fluoromount mounting media (Product No. F4680) contain an antifade reagent?
1 respuesta-
Fluoromount does not contain an antifade reagent. If you need an antifade mounting media, check Fluoroshield (Product Nos. F6182, F6057 and F5932) or PVA with antifade (Product Nos. 10981 and 10979).
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How does the storage temperature relate to shipping conditions?
1 respuesta-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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How do you remove the coverslip after using Fluoromount (Product No. F4680)?
1 respuesta-
Place the slide in a beaker full of deionized water on a magnetic stirrer. Gently stir the slide for a few hours to overnight for complete removal of dry Fluoromount.
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Does Fluoromount mounting media (Product No. F4680) contain glycerol?
1 respuesta-
Fluoromount does not contain glycerol. The exact formulation is considered proprietary.
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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