FD4

Sigma-Aldrich

Fluorescein isothiocyanate–dextran

average mol wt 3,000-5,000

Sinónimos:
FITC–Dextran
Número de CAS:
Número MDL:
NACRES:
NA.25

Nivel de calidad

100

origen biológico

synthetic

formulario

powder

mol peso

average mol wt 3,000-5,000

extensión del etiquetado

0.001-0.020 mol FITC per mol glucose

solubilidad

H2O: 25 mg/mL

conjugado

FITC conjugate

temp. de almacenamiento

2-8°C

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Descripción general

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Aplicación

FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. 5kDa FITC-dextran may be used to calibrate microscale diffusion measurements. 5kDa FITC-dextran may be used to measure gut permeability in gut barrier dysfunction research.
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

Nota de preparación

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

hazcat

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

storage_class_code

11 - Combustible Solids

WGK Alemania

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificado de Análisis

Certificado de origen

Hans Vergauwen et al.
Journal of animal science and biotechnology, 8, 30-30 (2017-04-14)
In this study the physiological implications of artificial rearing were investigated. Low (LBW) and normal birth weight (NBW) piglets were compared as they might react differently to stressors caused by artificial rearing. In total, 42 pairs of LBW and NBW...
Pratibha Singh et al.
Disease models & mechanisms, 13(8) (2020-08-06)
Prematurity and enteral feedings are major risk factors for intestinal injury leading to necrotizing enterocolitis (NEC). An immature digestive system can lead to maldigestion of macronutrients and increased vulnerability to intestinal injury. The aim of this study was to test...
Nathan J Klingensmith et al.
Shock (Augusta, Ga.), 50(2), 178-186 (2017-10-13)
Honokiol is a biphenolic isolate extracted from the bark of the magnolia tree that has been used in traditional Chinese and Japanese medicine, and has more recently been investigated for its anti-inflammatory and antibacterial properties. Honokiol has previously been demonstrated...
Akihiro Watari et al.
Scientific reports, 7(1), 14514-14514 (2017-11-08)
Claudins are key functional and structural components of tight junctions (TJs) in epithelial cell sheets. The C-terminal fragment of Clostridium perfringens enterotoxin (C-CPE) binds to claudin-4 and reversibly modulates intestinal TJ seals, thereby enhancing paracellular transport of solutes. However, the...
Jing Wang et al.
Frontiers in microbiology, 9, 1953-1953 (2018-09-11)
Weaning disturbs the intestinal barrier function and increases the risk of infection in piglets. Probiotics exert beneficial health effects, mainly by reinforcing the intestinal epithelium and modulating the gut microbiota. However, the mechanisms of action, and especially, the specific regulatory...
Artículos
Additional information about FITC-labelled polysaccarides including FITC-Dextran, FITC-DEAE-dextran (FDD), FITC-carboxymethyl-dextran (FCM-Dextran). This page discusses the impact of weight-average molecular weight on the respective molecule dimensions and other physical properties
Más información
Contenido relacionado
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
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