HSOLIGOINT
CRISPR Integration Kit
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
6 vials | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 510,00 € |
Acerca de este artículo
NACRES:
NE.02
UNSPSC Code:
12352200
Storage temp.:
−20°C
Shipped in:
dry ice
510,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarlerecombinant
expressed in E. coli
Quality Segment
packaging
pkg of (6 vials per kit)
application(s)
CRISPR
CRISPR
genome editing
selection
kanamycin
shipped in
dry ice
storage temp.
−20°C
General description
The CRISPR Integration Kit provides the essential genome editing reagents necessary to integrate a BstNI restriction site to the human KRAS locus. This kit is intended to serve as a tool to evaluate cell lines for ability to integrate new sequences at CRISPR-effected double strand breaks with the aid of short oligonucleotide donor. Integration efficiencies obtained with this kit enable rational design of genome editing experiments in a wide range of cell lines.
Application
CRISPR/Cas9 can be used to introduce, or “knock in”, new DNA sequences. Common modifications include the introduction of a SNP, small tag, loxP or larger cassette such as fluorescent protein. These modifications are made through the introduction of targeted DSB which enhance targeted integration significantly (Choulika et al., 1995). Targeted integration (gene knock in) occurs through homology directed repair (HDR)( (Bibikova et al., 2002). In order to enable gene editing by HDR, a DNA ‘donor′ or repair template containing the desired sequence must be delivered to the cell along with gRNA and Cas9.
Features and Benefits
- The CRISPR Integration Assay provides a validated CRISPR that cleaves DNA with high efficiency along with a standardized oligo donor designed to survey homology dependent repair frequencies in a wide variety of cell lines
- CRISPR-aided integration increases the targeted mutagenesis rate without the need for antibiotic selection
- Integration efficiencies estimated with this kit enable rational design of genome editing experiments in a wide range of cell lines
Other Notes
Each kit consists of:
one vial of gRNA plasmid - KRAS
one vial of Cas9 GFP plasmid
one vial of 120 base donor oligonucleotide
one vial of forward PCR primer
one vial of reverse PCR primer
one vial of genomic DNA control
one vial of gRNA plasmid - KRAS
one vial of Cas9 GFP plasmid
one vial of 120 base donor oligonucleotide
one vial of forward PCR primer
one vial of reverse PCR primer
one vial of genomic DNA control
Legal Information
1 of 1
Este artículo | |||
|---|---|---|---|
| description - | description - | description Design and order CRISPR gRNA, Cas9, screening libraries, controls and companion products. Formats include plant, lentivirus, IVT-RNA, plasmid, synthetic, and protein. | description - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| packaging pkg of (6 vials per kit) | packaging vial of 50 μL | packaging - | packaging vial of 50 μL |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| selection kanamycin | selection - | selection - | selection - |
Clase de almacenamiento
10 - Combustible liquids
WGK
nwg
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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