LIG2
QuickLink™ DNA Ligation Kit
joins blunt-end and sticky-end DNA fragments
Sinónimos:
DNA Ligation Kit, Ligation Kit
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 557,00 € |
Acerca de este artículo
UNSPSC Code:
12352200
EC Number:
232-770-0
NACRES:
NA.55
557,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlegrade
Molecular Biology
Quality Segment
form
liquid
usage
kit sufficient for 50 ligation reactions
technique(s)
molecular cloning: suitable
shipped in
dry ice
storage temp.
−70°C
General description
Sigma′s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions at room temperature using blunt or sticky ends. This kit replaces previous workflows requiring cooking steps and long incubations. Pre-made buffers allow for fast & easy setup.
Application
QuickLink™ DNA Ligation Kit has been used for the ligation of pGL2-basic luciferase-reporter plasmid with vascular endothelial growth factor (VEGF) promoter sequence and for the ligation of NLR family caspase activating and recruitment domain (CARD)-containing protein 4 (Nlrc4) promoter with the luciferase plasmid vector pGL4.10-luc2
The QuickLink™ DNA Ligation Kit is suitable for:
- Joining blunt or cohesive-end fragments of DNA into a cloning vector
- Recircularization of linear DNA
- Formation of concatamers
- dsDNA nick repair
Biochem/physiol Actions
One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA.
Features and Benefits
- Fast 5 minutes ligation
- High ligation efficiency
- Room temperature reactions – no cooling required
- Perform bacterial transformation with the reaction mixture
Other Notes
Sufficient for 50 reactions:
- 500uL 2X Ligation Buffer A (L9537)
- 100uL 5X Ligation Buffer B (L9662)
- 250 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM DTT
Legal Information
QuickLink is a trademark of Sigma-Aldrich Co. LLC
1 of 1
Este artículo | |||
|---|---|---|---|
| description joins blunt-end and sticky-end DNA fragments | description - | description - | description Dependable ligation with maximum flexibility |
| usage kit sufficient for 50 ligation reactions | usage 1 kit sufficient for 150 ligation reactions | usage kit sufficient for 100 assays | usage - |
| technique(s) molecular cloning: suitable | technique(s) - | technique(s) - | technique(s) - |
| storage temp. −70°C | storage temp. −20°C | storage temp. −70°C | storage temp. - |
| form liquid | form - | form - | form - |
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Clase de almacenamiento
10 - Combustible liquids
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
Palabra de advertencia
Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Resp. Sens. 1
¿Qué está pasando?
WGK 1
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.