Really good value for money and always arrives with a good shelf-life in terms of expiry date. Great for adding cytokines + hydrocortisone to to create your own endothelial cell growth medium - has kept my HUVECs consistently happy since I started using it in 2018. Also great to use when isolating human primary endothelial cells.
M4530
Medium 199
With Earle′s salts, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
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Sinónimos:
M199 Medium, TCM 199
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
100 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 15,50 € |
500 mL | Disponible para enviar HOYdesdeLyon | 26,00 € |
1 L | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 46,80 € |
Acerca de este artículo
UNSPSC Code:
12352207
NACRES:
NA.75
15,50 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleNombre del producto
Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- as a suspension media to maintain the primary neuronal cultures
- as a component of the collagen gel for ex vivo embryonic heart culture
- to maintain the ovarian tissue sections as part of the slow-freezing protocol
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Este artículo | |||
|---|---|---|---|
| description With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | description With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | description HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture | description Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture |
| form liquid | form liquid | form powder | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility - |
| components NaHCO3: yes | components sodium pyruvate: no | components L-glutamine: yes | components L-glutamine: no |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
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Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Listados normativos
Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.
64-17-5
CAS No.
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