MTOXCE2B6

CypExpress^™ 2B6 Cytochrome P450 human

• NACRES: NA.77
• UNSPSC Code: 12352200
• Form: dry powder
• Biological source: human
• Recombinant: expressed in Pichia pastoris

Propiedades

• biological source: human
• recombinant: expressed in Pichia pastoris
• form: dry powder
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CYP2B6(1555)

Descripción

Biochem/physiol Actions

CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.

Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous.

Features and Benefits

CypExpress 2B6 is a permeabilized and stabilized dried yeast powder preparation containing full length, unmodified, human CYP2B6 and recombinant human NADPH oxidoreductase. Using proprietary processes, CypExpress are expressed in Pichia yeast. Following optimal expression, the cells are inactivated, permeabilized and dried to a fine powder. CypExpress retains the cellular mechanisms to provide the P450 enzymes with the energy and cofactors to continue to function for long experiments, and can generate larger amounts of metabolite than mammalian microsomes or other genetically engineered expression systems.It is designed for rapid screening of drug metabolites and for scaling up metabolite generation.

Legal Information

CypExpress is a trademark of Oxford Biomedical Research

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• ¿Qué está pasando?: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable