OGS51

PSF-U6 - SIRNA/RNAI EXPRESSION PLASMID

plasmid vector for molecular cloning

• Sinónimos: cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• NACRES: NA.85
• UNSPSC Code: 12352200
• Promoter: Promoter name: U6; Promoter activity: siRNA; Promoter type: mammalian
• Origin of replication: pUC (500 copies)
• Bacteria selection: kanamycin
• Reporter gene: none
• Peptide cleavage: no cleavage

Propiedades

• form: buffered aqueous solution
• mol wt: size 3932 bp
• bacteria selection: kanamycin
• origin of replication: pUC (500 copies)
• peptide cleavage: no cleavage
• promoter: Promoter name: U6; Promoter activity: siRNA; Promoter type: mammalian
• reporter gene: none
• shipped in: ambient
• storage temp.: −20°C

Descripción

General description

The expression of short hairpin RNA in eukaryotic cells. The U6 is transcribed by RNA polymerase III (RNA pol III) which terminates transcription at a string of thymidine residues typically a minimum of five residues in mammalian cells. Unlike most of our vectors, this vector contains an RNA pol III terminator downstream of the XhoI site although it is more common to insert a terminator sequence at the end of the sequence you insert into the MCS that encodes a shRNA.

Promoter Expression Level:

Application

Multiple cloning site notes: This vector has a modified MCS compared to standard plasmids. The ATG start codon in the NcoI site has been removed as have the Shine-Dalgarno and KOZAK sequences as these are not required for RNA Pol3 expressed transcripts.

The terminator for RNA polymerase III has been positioned immediately downstream of the XhoI site. There is an additional string of Thymidine residues (the terminator sequence for RNA Pol III) at the end of the SV40 polyA region however using this site will produce a long transcript and has not been validated. We recommend inserting your hairpin DNA sequence between Hind3 and XhoI. The theoretical RNA position +1 is the first base of the NotI restriction site.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Other Notes

To view sequence information for this product, please visit the product page

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable