The Brunello CRISPR library utilizes puromycin selection.
PCRISPR007
Human CRISPR Brunello Knockout Library
Sinónimos:
CRISPR Brunello Library, CRISPR Knockout Library, Knockout Library Kit
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
5 x 200 μL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 7390,00 € |
Acerca de este artículo
NACRES:
NE.02
UNSPSC Code:
41105904
7390,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlepackaging
pkg of 5 vials (5x200µL aliquots )
concentration
≥5x108 VP/ml (via p24 Assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
The human CRISPR ′Brunello′ pooled library is designed using optimized metrics, as published by, Doench et al. Nat Biotechnol. (2016) and described further in Sanson, K.R., et al. Nat Commun (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Application
- Functional Genomics/Target Validation
- Unbiased wholed genome forward genetic screening
- Validated positive and negative controls
- Set up and optimization of screen assay
Biochem/physiol Actions
In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by a gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.
Features and Benefits
- Focus on your research, and we will generate your lentivirus screening library
- Use CRISPR nucleases to knockout protein-coding genes to assess their function.
- Human Genes targeted 19,114
- Compact library of ~ four gRNAs per gene (76,441 total)
- Total Controls 1000(pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO for sources of Cas9
1 of 1
Este artículo | |||
|---|---|---|---|
| description - | description - | description - | description - |
| application(s) CRISPR | application(s) CRISPR | application(s) CRISPR | application(s) CRISPR |
| packaging pkg of 5 vials (5x200µL aliquots ) | packaging pkg of 10 vials (5x200µL aliquots of each component) | packaging pkg of 5 vials (5x200µL aliquots ) | packaging pkg of 5 vials (5x200µL aliquots ) |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| concentration ≥5x108 VP/ml (via p24 Assay) | concentration ≥5x108 VP/ml (via p24 Assay) | concentration ≥5x108 VP/ml (via p24 Assay) | concentration ≥5x108 VP/ml (via p24 Assay) |
Clase de almacenamiento
10 - Combustible liquids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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What selection marker is included in the lentiviral library?
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