The presence of urea can reduce the depletion of albumin with PROTIA. We would suggest a buffer exchange prior to depletion using either dialysis or 5 KDa NMWL cut-off spin filters.
PROTIA
ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit
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Código UNSPSC:
41106500
NACRES:
NA.56
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immunodepletion: suitable
temp. de almacenamiento
2-8°C
1 of 4
Este artículo | PROTMEM | PROTDT | PROTBA |
|---|---|---|---|
| technique(s) immunodepletion: suitable | technique(s) - | technique(s) - | technique(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Descripción general
ProteoPrep® Immunoaffinity Albumin and IgG Depletion Kit might be a useful tool for novel biomarker discovery.[1]
The ProteoPrep® Immunoaffinity medium in the prepacked spin columns is a mixture of two-beaded media containing recombinantly expressed, small single-chain antibody ligands, resulting in low non-specific binding and high capacity. The ProteoPrep Immunoaffinity Albumin and IgG Depletion Kit has been designed to specifically remove albumin and IgG from human serum (25-50μL) in preparation of samples for proteomics analysis, two-dimensional electrophoresis (2DE) or liquid chromatography (LC). This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum.
Aplicación
Características y beneficios
- High Capacity removes more interfering abundant proteins
- High Specifity ensures you see an accurate and more complete proteome
- Buffer systems are compatible with 2 dimensional electrophoresis so there are no intermediate time consuming steps that might result in protein loss
Información legal
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1
Código de clase de almacenamiento
10 - Combustible liquids
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High abundance protein depletion is a major challenge in the study of serum/plasma proteomics. Prior to this study, most commercially available kits for depletion of highly abundant proteins had only been tested and evaluated in adult serum/plasma, while the depletion
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My protein samples contain urea. Can I still use the ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, Product PROTIA, for depletion?
1 respuesta-
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Can I use the ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, Product PROTIA, on samples besides serum?
1 respuesta-
Besides human serum or plasma samples, we have not specifically tested other human biological fluids with this kit. However, in principle, there is no reason not to try the kit with other fluid samples.
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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