This kit has been optimized with cell lysate and tissue lysate as the input sample types. If you are interested in a kit that can be used for cell culture supernatants, we recommend RAB0507 which is designed for serum, plasma, cell culture supernatants and urine samples.
RAB0508
Human VEGF ELISA Kit
for cell and tissue lysates
Sinónimos:
VEGF-A, Vascular endothelial growth factor A
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 569,00 € |
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NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... VEGFA(7422)
Input:
sample type cell lysate
sample type tissue lysate
sample type tissue lysate
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
569,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarlespecies reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type cell lysate
sample type tissue lysate
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... VEGFA(7422)
General description
The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in cell lysate and tissue lysate.
Immunogen
Recombinant Human VEGF-A
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis. Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes. Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease. Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves′ disease, psoriasis, cardiovascular disease, and peripheral limb disease.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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Este artículo | |||
|---|---|---|---|
| description for cell and tissue lysates | description for serum, plasma, cell culture supernatants and urine | description for serum, plasma, cell culture supernatant | description for cell and tissue lysates |
| species reactivity human | species reactivity human | species reactivity mouse | species reactivity mouse |
| Gene Information human ... VEGFA(7422) | Gene Information human ... VEGFA(7422) | Gene Information mouse ... Vegfa(22339) | Gene Information mouse ... Vegfa(22339) |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% |
| input sample type cell lysate | input sample type plasma | input sample type cell culture supernatant(s) | input sample type cell lysate |
| technique(s) capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
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Warning
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Met. Corr. 1
Clase de almacenamiento
10 - Combustible liquids
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can this kit be used for cell culture supernatants as well? if the same sensitivity is required and if the lysis buffer is not used...
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