The principal of this kit is based on chemical binding and elution rather than size exclusion. To recover more of the small-sized RNA, an increase the amount of Binding Solution is suggested, however some small RNA molecules, including microRNA, siRNA, tRNA, and 5S rRNA, are not efficiently recovered. To maximize the recovery of small RNAs, refer to Protocol A on page 5 of the user guide – link provided below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/136/spectrumrnakitug-ms.pdf
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 364,00 € |
Acerca de este artículo
364,00 €
usage
sufficient for 50 purifications
Quality Segment
sustainability
Sustainability features
technique(s)
RNA purification: suitable
test parameters
: ~30 min kit run time, sample volume: 100 mg
storage temp.
15-25°C
General description
Después de moler el tejido hasta un polvo fino en nitrógeno líquido, se lisan las células, y los restos celulares se separan física y químicamente del ARN endógeno. A continuación el ARN se une a un sustrato de sílice sustentado en columna y varias etapas de lavado eliminan los contaminantes remanentes.
El ARN total es eluido de la columna y utilizado en las aplicaciones habituales como inmunotransferencias Northern, RT-PCR y qRT-PCR.
Application
Features and Benefits
- Produce hasta 60 μg de ARN concentrado puro por preparación
- Protocolo eficiente que permite la purificación del ARN en 30 minutos o menos
- Especialmente diseñado para la investigación con tejidos vegetales difíciles
Legal Information
1 of 1
Este artículo | |||
|---|---|---|---|
| description sufficient for 50 purifications | description sufficient for 250 purifications | description sufficient for 10 purifications | description Suitable for any plant species or tissue |
| technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) protein extraction: suitable |
| usage sufficient for 50 purifications | usage sufficient for 250 purifications | usage sufficient for 10 purifications | usage - |
| greener alternative category | greener alternative category | greener alternative category , Aligned | greener alternative category - |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. −20°C |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics - |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability - |
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Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
Órganos diana
Liver,Heart
Clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
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Which is the exclusion size for RNAs purified with this kit ? I am wondering in the retention size of small RNAs
1 respuesta-
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What composition of your elution buffer (E8024-50ml) in plant total RNA kit?
1 respuesta-
The composition of this product is considered proprietary.
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Is it suitable for use with chestnut samples?
Is it suitable for use with chestnut samples?
1 respuesta-
The kit has been utilized for chestnut samples, as reported in the following paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615883/
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We need to know the elution buffer composition for downstream quantification and sequencing. What solution can be used besides the original elution buffer that can have the same proprerties to be used as a blank?
1 respuesta-
A suitable solution would be molecular biology-grade water or nuclease-free water. These types of water are typically free of contaminants and nucleases, making them ideal for use as a blank solution in downstream quantification and sequencing experiments.
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