TRUE9
TrueGel3D Hydrogel Kits
SLO-PVA, PEG non cell-degradable crosslinker
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 89,20 € |
Acerca de este artículo
NACRES:
NA.71
UNSPSC Code:
12352207
Storage temp.:
−70°C
Shipped in:
dry ice
89,20 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleapplication(s)
3D cell culture
shipped in
dry ice
storage temp.
−70°C
General description
The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-PVA. The polymer, SLO-PVA is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-PVA. SLO-PVA does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.
Application
TRUE9-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of matrix stiffness on cell physiology and behavior.SLO-PVA based gels are used to grow cells that do not need attachment to hydrogel for survival but rather use their secreted extracellular matrix. The SLO-PVA based hydrogels are preferred over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes. TrueGel3DTM Hydrogel Kits has been used in the generation of 3D gel based tumor models.
Features and Benefits
- Slow gelling (takes 6 minutes to 25 minutes, depends on gel stiffness)
- Flexibility in modulating gel stiffness
- Does not allow cell migration
- Does not allow cell recovery and post-culture analyses
- Cell inert hydrogel (if not customized with RGD peptide)
Other Notes
TRUE9-1KT contains SLO-PVA solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.
Disclaimer
Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store SLO-PVA in small portions to avoid frequent freeze-thaw cycles.
1 of 1
Este artículo | |||
|---|---|---|---|
| description SLO-PVA, PEG non cell-degradable crosslinker | description FAST-PVA, CD cell-degradable crosslinker | description SLO-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker | description SLO-PVA, CD cell-degradable crosslinker |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
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Warning
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
¿Qué está pasando?
WGK 3
Clase de almacenamiento
10 - Combustible liquids
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