- Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes.
Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes.
STAR protocols (2020-10-29)
Mandy Kleinsorge, Lukas Cyganek
PMID33111079
RESUMEN
The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient monolayer-based differentiation of hiPSCs into defined atrial and ventricular cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Cyganek et al., 2018.
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