An enzyme-responsive nanocontainer as an intelligent signal-amplification platform for a multiple proteases assay.

An enzyme-responsive nanocontainer as an intelligent signal-amplification platform for a multiple proteases assay.

Chemical communications (Cambridge, England) (2012-10-10)

Xinjian Yang, Fang Pu, Cuie Chen, Jinsong Ren, Xiaogang Qu

RESUMEN

Based on selective pore-opening in the presence of protease, we have developed a novel signal amplification assay for multiple proteases detection and their inhibition using protein-capped mesoporous scaffolding as the substrate.

MATERIALES

Número de producto

Marca

Descripción del producto

P7626

Sigma-Aldrich

Fluoruro de fenilmetansulfonilo, ≥98.5% (GC)

G7141

Sigma-Aldrich

Glucosa oxidasa from Aspergillus niger, Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)

G2133

Sigma-Aldrich

Glucosa oxidasa from Aspergillus niger, Type VII, lyophilized powder, ≥100,000 units/g solid (without added oxygen)

93482

Sigma-Aldrich

Phenylmethanesulfonyl fluoride solution, ~0.1 M in ethanol (T)

78830

Sigma-Aldrich

Fluoruro de fenilmetansulfonilo, ≥99.0% (T)