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Merck

Efficacy of pentane, toluene, and benzene to support aerobic cometabolism of ethylene dibromide.

New biotechnology (2012-05-23)
Anthony S Danko, Patrícia O Leitão, Matthew F Verce, David L Freedman
RESUMEN

The ability of pentane, benzene, and toluene to support aerobic cometabolism of ethylene dibromide (1,2-dibromoethane, EDB) was evaluated. A pentane enrichment culture cometabolized EDB, with a transformation capacity of 0.35 μmol EDB/mg biomass (66.2 μg EDB/mg biomass) in the absence of growth substrate. It also cometabolized EDB while actively growing on pentane. However, enrichment cultures grown on benzene or toluene could not cometabolize EDB, with or without their respective growth substrates.

MATERIALES
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Sigma-Aldrich
1,2-Dicloroetano, ACS reagent, ≥99.0%
Sigma-Aldrich
1,2-Dicloroetano, anhydrous, 99.8%
Sigma-Aldrich
Pentane, ≥99% (GC)
Sigma-Aldrich
1,2-Dibromoethane, 98%
Sigma-Aldrich
1,2-Dibromoethane, ≥99%
Supelco
1,2-Dicloroetano, analytical standard
Supelco
1,2–Dichloroethane solution, certified reference material, 200 μg/mL in methanol
Sigma-Aldrich
1,2-Dibromoethane, purum, ≥98.0% (GC)
Supelco
1,2-Dibromoethane solution, certified reference material, 200 μg/mL in methanol
Supelco
Pentane, analytical standard