Biodegradation of tetrahydrofuran and 1,4-dioxane by soluble diiron monooxygenase in Pseudonocardia sp. strain ENV478.

Biodegradation of tetrahydrofuran and 1,4-dioxane by soluble diiron monooxygenase in Pseudonocardia sp. strain ENV478.

Journal of molecular microbiology and biotechnology (2012-11-14)

Hisako Masuda, Kevin McClay, Robert J Steffan, Gerben J Zylstra

RESUMEN

1,4-Dioxane is an important groundwater contaminant. Pseudonocardia sp. strain ENV478 degrades 1,4-dioxane via cometabolism after the growth on tetrahydrofuran (THF) and other carbon sources. Here, we have identified a THF monooxygenase (thm) in ENV478. The thm genes are transcribed constitutively and are induced to higher levels by THF. Decreased translation of the thmB gene encoding one of the monooxygenase subunits by antisense RNA resulted in the loss of its ability to degrade THF and 1,4-dioxane. This is the first study to link thm genes to THF degradation, as well as the cometabolic oxidation of 1,4-dioxane.

MATERIALES

Número de producto

Marca

Descripción del producto

34865

Sigma-Aldrich

Tetrahidrofuran, inhibitor-free, suitable for HPLC, ≥99.9%

360589

Sigma-Aldrich

Tetrahidrofuran, contains 200-400 ppm BHT as inhibitor, ACS reagent, ≥99.0%

401757

Sigma-Aldrich

Tetrahidrofuran, anhydrous, ≥99.9%, inhibitor-free

360481

Sigma-Aldrich

1,4 Dioxano, ACS reagent, ≥99.0%, contains ≤25 ppm BHT as stabilizer

34857

Sigma-Aldrich

1,4 Dioxano, suitable for HPLC, ≥99.5%

676934

Sigma-Aldrich

1,4 Dioxano, ACS reagent, ≥99.0%, contains <=25 ppm BHT as stabilizer

676764

Sigma-Aldrich

Tetrahidrofuran, ACS reagent, ≥99.0%, contains 200-400 ppm BHT as inhibitor

76887

Supelco

1,4 Dioxano, analytical standard

CRM48367

Supelco

1,4-Dioxane solution, certified reference material, 2000 μg/mL in methanol, ampule of 1 mL

78445

Supelco

Tetrahidrofuran, analytical standard

551368

Sigma-Aldrich

1,4-Dioxane solution, suitable for NMR (reference standard), 40% in benzene-d₆ (99.6 atom % D)

87369

Supelco

Tetrahidrofuran, Selectophore^™, ≥99.5%

736171

Supelco

1,4-Dioxane solution, suitable for NMR (reference standard), 40% in benzene-d₆ (99.6 atom % D), NMR tube size 5 mm × 8 in.

704814

Sigma-Aldrich

1,4-Dioxane solution, suitable for NMR (reference standard), 10 mM in D₂O ("100%", 99.96 atom % D), NMR tube size 5 mm × 7 in.

684228

Sigma-Aldrich

1,4-Dioxane solution, suitable for NMR (reference standard), 40% in benzene-d₆ (99.6 atom % D), NMR tube size 10 mm × 8 in.

612073

Sigma-Aldrich

1,4-Dioxane solution, suitable for NMR (reference standard), 40% in benzene-d₆ (99.6 atom % D), chromium(III) acetylacetonate 5 mg/mL, NMR tube size 3 mm × 8 in.

611905

Sigma-Aldrich

1,4-Dioxane solution, suitable for NMR (reference standard), 40% in benzene-d₆ (99.6 atom % D), chromium(III) acetylacetonate 5 mg/mL, NMR tube size 5 mm × 8 in.

631868

Supelco

1,4-Dioxane solution, suitable for NMR (reference standard), 5 mM in chloroform-d (99.8 atom % D), NMR tube size 3 mm × 8 in.

631833

Supelco

1,4-Dioxane solution, suitable for NMR (reference standard), 5 mM in benzene-d₆ (99.6 atom % D), NMR tube size 3 mm × 8 in.