- DNA duplex stabilization in crowded polyanion solutions.
DNA duplex stabilization in crowded polyanion solutions.
Chemical communications (Cambridge, England) (2013-01-11)
Imran Khimji, Jeehae Shin, Juewen Liu
PMID23302897
RESUMEN
The melting temperature of duplex DNA is much higher in polyanions than in non-ionic polymers with similar ionic strength, suggesting an additional electrostatic contribution on top of the excluded volume effect.
MATERIALES
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Sigma-Aldrich
Poly(acrylic acid) solution, average Mw ~2,000, 50 wt. % in H2O, electronic grade
Supelco
Poly(acrylic acid sodium salt), analytical standard, suitable for gel permeation chromatography (GPC), 16,000
Supelco
Poly(acrylic acid sodium salt), analytical standard, suitable for gel permeation chromatography (GPC), 1,250