Validated high-performance liquid chromatographic method for quantitation of neohesperidine dihydrochalcone in foodstuffs.

An analytical method to detect and quantitate neohesperidine dihydrochalcone in foodstuffs has been developed and validated in soft-drink applications. The method was shown to be sufficiently precise, accurate, selective and rugged in quantitating neohesperidine DC both at flavouring (1-5 mg/kg) and sweetening (5-50 mg/kg) levels. Applications of the method to determine neohesperidine DC in foodstuffs other than soft-drinks is also described.