Mucor hiemalis endo-beta-N-acetylglucosaminidase can transglycosylate a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide.

Mucor hiemalis endo-beta-N-acetylglucosaminidase can transglycosylate a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide.

Carbohydrate research (2004-10-13)

Kenji Osumi, Yoshitaka Makino, Eri Akaike, Takashi Yamanoi, Mamoru Mizuno, Midori Noguchi, Toshiyuki Inazu, Kenji Yamamoto, Kiyotaka Fujita

PMID15476727

RESUMEN

We found that the recombinant endo-beta-N-acetylglucosaminidase of Mucor hiemalis (Endo-M) expressed in Candida boidinii had the transglycosylation activity of transferring a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide to the acceptor (p-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside) in a good yield of 43%.

MATERIALES

Número de producto

Marca

Descripción del producto

N9376

Sigma-Aldrich

4-Nitrophenyl N-acetyl-β-D-glucosaminide, ≥99% (TLC)

En este momento no podemos mostrarle ni los precios ni la disponibilidad

N8759

Sigma-Aldrich

4-Nitrophenyl N-acetyl-α-D-glucosaminide, ≥98%

En este momento no podemos mostrarle ni los precios ni la disponibilidad