Synthesis of S-farnesyl-L-cysteine methylester and purification by HPLC.

S-trans, trans-farnesyl-L-cysteine methylester, a post translational modified amino acid, was synthesized from farnesyl bromide and L-cysteine methylester hydrochloride salt in the presence of triethylamine. Its purification as well as separation from the other isomers by HPLC on RP Vydac C4 and C8 columns are reported here.