Impact of germination on the structures and in vitro digestibility of starch from waxy brown rice.

International journal of biological macromolecules (2015-11-20)
Su-Yeon You, Sea-Gwan Oh, Hye Min Han, Wujin Jun, Young-Shick Hong, Hyun-Jung Chung
RESUMEN

The in vitro digestibility as well as the molecular and crystalline structures of waxy rice starches isolated from brown rice, germinated brown rice (GBR), ultrasonicated GBR, and heat-moisture treated GBR were investigated. The germinated brown rice starch (GBRS) had a lower average molecular weight and a higher proportion of DP 6-12 in amylopectin than brown rice starch (BRS). The relative crystallinity, intensity ratio of the band at 1,047 cm(-1) and 1,022 cm(-1), gelatinization temperature and pasting temperature of waxy rice starch were reduced by germination. However, the ultrasonication and heat-moisture treatment of GBRS increased the relative crystallinity and gelatinization temperature. The digestibility of starch from brown waxy rice was increased by germination. The rapidly digestible starch (RDS), slowly digestible starch (SDS) and resistant starch (RS) contents were 50.5%, 42.4%, and 7.1% in BRS, and 69.0%, 27.9% and 3.1% in GBRS, respectively. The ultrasonication and heat-moisture treatment of GBRS reduced RDS content and increased RS content in raw and gelatinized starches. The decrease in starch digestibility of cooked GBR was more pronounced after heat-moisture treatment than after ultrasonication.

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Supelco
Amyloglucosidase solution from Aspergillus niger, for use in Total Dietary Fiber Assay, TDF-100A
Sigma-Aldrich
Pancreatin from porcine pancreas, 8 × USP specifications