Merck
  • Home
  • Search Results
  • Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates.

Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates.

PloS one (2017-04-05)
Rebeca M Torrente-Rodríguez, Víctor Ruiz-Valdepeñas Montiel, Susana Campuzano, María Pedrero, Meryem Farchado, Eva Vargas, F Javier Manuel de Villena, María Garranzo-Asensio, Rodrigo Barderas, José M Pingarrón
ABSTRACT

The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
IgG from human serum, reagent grade, ≥95% (HPLC), buffered aqueous solution
Sigma-Aldrich
Hemoglobin human, lyophilized powder