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4B200

Sepharose™ 4B

45-165 μm bead diameter

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About This Item

CAS Number:
9012-36-6
MDL number:
MFCD08457579
UNSPSC Code:
12352200
PubChem Substance ID:
24873317
NACRES:
NA.21

Key Documents

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biological source

algae (marine)

Quality Level

200

form

suspension

technique(s)

affinity chromatography: suitable

bead diameter

45-165 μm

pore size

30,000-50,000 Da fractionation range (Dextrans)
60,000-20,000,000 Da fractionation range (Globular proteins)

storage temp.

2-8°C

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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1 of 4

This Item
6B100121852A2576
Sepharose™ 4B 45-165 μm bead diameter

Sigma-Aldrich

4B200

Sepharose™ 4B

Sepharose™ 6B 6% Beaded Agarose, 45-165 μm (wet), fractionation range 10,000-1,000,000 Da (dextrans)

Sigma-Aldrich

6B100

Sepharose™ 6B

Agarose, Type C Highly purified non-ionic fraction of agar. Forms gel with superior mechanical qualities at very low concentrations.

Millipore

121852

Agarose, Type C

Agarose Ultra-low Gelling Temperature, molecular biology grade

Sigma-Aldrich

A2576

Agarose

technique(s)

affinity chromatography: suitable

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

electrophoresis: suitable

biological source

algae (marine)

biological source

algae (marine)

biological source

-

biological source

-

Quality Level

200

Quality Level

-

Quality Level

100

Quality Level

200

form

suspension

form

suspension

form

solid

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

-

bead diameter

45-165 μm

bead diameter

-

bead diameter

-

bead diameter

-

General description

Sepharose™ 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.[1]

Application

Sepharose™ 4B has been used:
  • to conjugate scorpion venom to be used in affinity chromatography[2]
  • for clearing of protein extracts prior to immunoprecipitation[3]
  • for gel purification and desalting of phospholipid vesicles[4]
  • for adsorption of immunoglobulin G (IgG)[5]

Legal Information

Sepharose is a trademark of Cytiva

comparable product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F - closed cup

Flash Point(C)

38 - 43 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ9618
MKCZ8546
MKCZ3155
MKCX1071
MKCZ1415

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Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations
Adamala K, et al.
Nature Protocols, 10(6), 927-927 (2015)
Oral Absorption of Mesobuthus eupeus Scorpion Venom in Mice.
Hosseini Z, et al.
Iranian Journal of Toxicology, 11(2), 15-19 (2017)
Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
Paul Kaufmann et al.
PloS one, 14(8), e0220866-e0220866 (2019-08-08)
Dipeptidyl amino-peptidase 3 (DPP3) is an aminopeptidase involved in peptide degradation, including hormone peptides as angiotensin II and enkephalins. DPP3 plasma activity increases in septic patients and correlates with mortality risk. However, the exact physiological role of DPP3 remains unclear
Barna Dey et al.
Journal of virology, 81(11), 5579-5593 (2007-03-16)
The human immunodeficiency virus type 1 exterior gp120 envelope glycoprotein is highly flexible, and this flexibility may contribute to the inability of monomeric gp120 immunogens to elicit broadly neutralizing antibodies. We previously showed that an S375W modification of a critical
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