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EMS0004

SOLu-Trypsin Protease

Ready-to-use stable solution for automation, suitable for mass spectrometry, recombinant, expressed in Pichia pastoris

Synonym(s):

Protein Digestion for Mass Spectrometry, Ready to Use Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Trypsin for Protein Digestion, rTrypsin

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100 μG

€81.00

4 X 100 μG

€300.00

€81.00

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.78

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Product Name

SOLu-Trypsin, recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid

recombinant

expressed in Pichia pastoris

grade

Proteomics Grade

description

Activity:≥ 10,000 unit/mg protein

form

ready-to-use solution

suitability

suitable for mass spectrometry

UniProt accession no.

P00761

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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SOLu-Trypsin recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid

Sigma-Aldrich

EMS0004

SOLu-Trypsin

SOLu-Trypsin Dimethylated Dimethlyated, Proteomics Grade, recombinant, expressed in Pichia pastoris, ready-to-use solution

Sigma-Aldrich

EMS0005

SOLu-Trypsin Dimethylated

SILu™Lite APOB48, Apolipoprotein B-48 human recombinant, expressed in HEK 293 cells, MS Protein Standard

Sigma-Aldrich

MSST0056

SILu™Lite APOB48, Apolipoprotein B-48 human

Recombinant Trypsin Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris

Sigma-Aldrich

EMS0006

Recombinant Trypsin

recombinant

expressed in Pichia pastoris

recombinant

expressed in Pichia pastoris

recombinant

expressed in HEK 293 cells

recombinant

expressed in Pichia pastoris

grade

Proteomics Grade

grade

Proteomics Grade

grade

-

grade

Proteomics Grade

suitability

suitable for mass spectrometry

suitability

suitable for mass spectrometry

suitability

suitable for mass spectrometry (internal calibrator)

suitability

suitable for , suitable for mass spectrometry

form

ready-to-use solution

form

ready-to-use solution

form

liquid

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

Application

Poster: Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
SOLu-Trypsin:
  • has been used to digest the gel pieces for mass spectrometry identification of active deubiquitnases (DUBs)[1]
  • has been used in trypsin sensitivity assay[2]
  • may be used for the digestion of wine proteins[3]

Biochem/physiol Actions

Trypsin is used in proteomics research[4] for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. It hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues.[5]
  • SOLu-Trypsin (EMS0004) is our exclusive, Advanced Proteomics Grade enzyme that is solution-stable for mass spectrometry.
  • Designed to be stable in solution when refrigerated, SOLu-Trypsin can be used immediately without preparation.
  • Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately.
  • SOLu-Trypsin allows excess product to be saved for future use, thus eliminating unnecessary waste and cost.
  • It is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion.

Features and Benefits

  • Ready to use - no preparation, such as reconstitution or thawing, is required
  • Fits seamlessly into established workflow - no need to modify protocols
  • Eliminates waste - remains stable in the refrigerator after use so there is no need to discard excess product
  • Recombinant, porcine sequence - no chymotryptic activity
  • Stable for short-term use at room temperature in an autosampler or on a liquid handling robot

General description

Trypsin is a pancreatic serine endoprotease. SOLu-Trypsin is made from recombinant trypsin, porcine sequence. It does not possess chymotryptic activity.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000508818
0000508819
0000508819
0000508818
0000498828

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Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications
Heissel S, et al.
PLoS ONE, 14 (2019)
Søren Heissel et al.
PloS one, 14(6), e0218374-e0218374 (2019-06-28)
Trypsin is by far the most commonly used protease in proteomics. Even though the amount of protease used in each experiment is very small, digestion of large amounts of protein prior to enrichment can be rather costly. The price of
The CHORD protein CHP-1 regulates EGF receptor trafficking and signaling in C. elegans and in human cells
Haag A, et al.
eLife, 9, e50986-e50986 (2020)
Mass spectrometry of peptides and proteins using digestion by a grape cysteine protease at pH 3
Perutka Z and Sebela M
Journal of Mass Spectrometry : Jms (2019)
Zdeněk Perutka et al.
Journal of mass spectrometry : JMS, 55(7), e4444-e4444 (2019-10-12)
Cysteine protease from grapevine (Vitis vinifera) belongs to those resistant proteins, which survive the process of vinification and can therefore be detected as wine components. Its amino acid sequence shows a homology to other members of the papain family, but
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