66540489
UKKi024-B
Human iPS Cell Line
Sign In to View Organizational & Contract Pricing.
About This Item
UNSPSC Code:
41106509
Biological source:
human peripheral blood
Relevant disease(s):
Brugada syndrome
Reprogramming method:
Sendai virus
Gender:
male
Growth mode:
adherent (pluripotent)
biological source
human peripheral blood
reprogramming method
Sendai virus
description
age (30-34)
manufacturer/tradename
EBiSC™
gender
male
growth mode
adherent (pluripotent)
technique(s)
cell culture | stem cell: suitable
relevant disease(s)
Brugada syndrome
shipped in
dry ice
storage temp.
−196°C
General description
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Biochem/physiol Actions
Depositor
Klinikum der Universität zu Köln
Klinikum der Universität zu Köln
Derivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
CD90 Yes -
NANOG Yes - -
FOXD3 Unknown -
TRA 1-80 Yes - -
SSEA-4 Yes - -
DNMT3b Unknown -
OCT 4 Yes - -
SSEA-1 Yes -
Morphology:
thawing control of NP0133-1
thawing control of NP0133-1
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0133-1
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0133-1
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0133-1
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 32
Cell line karyotype: No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
CD90 Yes -
NANOG Yes - -
FOXD3 Unknown -
TRA 1-80 Yes - -
SSEA-4 Yes - -
DNMT3b Unknown -
OCT 4 Yes - -
SSEA-1 Yes -
Morphology:
thawing control of NP0133-1
thawing control of NP0133-1
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0133-1
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0133-1
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0133-1
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 32
Cell line karyotype: No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Preparation Note
Medium: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -
Other Notes
Note: EAUA and CLIP must be completed before order fulfillment
Legal Information
EBiSC is a trademark of Fraunhofer-Gesellschaft
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
10108-64-2
CAS No.
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.