CC130

Human Vitronectin

Name: Human Vitronectin
Brand: MM
Price: 121 GBP
Availability: InStock

recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM^®

Synonym(s):

S-protein, Serum-spreading factor, V75

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500 μG

£121.00

Estimated to ship on01 December 2025Details

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About This Item

UNSPSC Code:

12352202

eCl@ss:

32160405

NACRES:

NA.75

Key Documents

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Product Name

PluriSTEM-XF^® Recombinant Vitronectin, Human pluripotent stem cell culture.

biological source

Escherichia coli
human

Quality Level

100

Assay

≥92% (SDS-PAGE)

form

liquid

manufacturer/tradename

PluriSTEM^®

concentration

0.5 mg/mL

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell culture | stem cell: suitable

input

sample type: human embryonic stem cell(s)

UniProt accession no.

P04004

shipped in

dry ice

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Show Differences

1 of 4

This Item	V0132	V8379	CC080
Sigma-Aldrich CC130 PluriSTEM-XF^® Recombinant Vitronectin	Sigma-Aldrich V0132 Vitronectin from rat plasma	Sigma-Aldrich V8379 Vitronectin from human plasma	Sigma-Aldrich CC080 Vitronectin, Human Purified Protein
biological source Escherichia coli, human	biological source rat plasma	biological source human plasma	biological source human
technique(s) cell attachment: suitable, cell culture \| stem cell: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
assay ≥92% (SDS-PAGE)	assay -	assay -	assay ≥90% (SDS-PAGE)
concentration 0.5 mg/mL	concentration -	concentration -	concentration 1 mg/mL
Quality Level 100	Quality Level 200	Quality Level 300	Quality Level 100
form liquid	form lyophilized powder	form lyophilized powder	form liquid

General description

PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.

Application

Research Category
Stem Cell Research

Cell Structure

ECM & Adhesion

Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.

Physical form

Product is supplied in a 20 mM pH 8.0 Tris-HCl buffer with a proprietary formulation of sucrose, EDTA, arginine, and DTT.

Preparation Note

Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles. Product has been 0.2 μm filtered.

Analysis Note

Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layers.

Mutsumi Kobayashi et al.

Stem cell reports, 17(3), 507-521 (2022-02-13)

In vitro expansion of human primordial germ cell-like cells (hPGCLCs), a pluripotent stem cell-derived PGC model, has proved challenging due to rapid loss of primordial germ cell (PGC)-like identity and limited cell survival/proliferation. Here, we describe long-term culture hPGCLCs (LTC-hPGCLCs), which

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