This antibody is compatible with enzyme immunoassay, which includes ELISA, the most widely used form of EIA.
F1804
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
Synonym(s):
flag antibody, Anti-ddddk, Anti-dykddddk
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50 μG
£147.00
200 μG
£489.00
1 MG
£1,240.00
5 MG
£1,810.00
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50 μG
£147.00
200 μG
£489.00
1 MG
£1,240.00
5 MG
£1,810.00
About This Item
UNSPSC Code:
12352203
NACRES:
NA.32
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
purified by
affinity chromatography (affinity tag purification)
species reactivity
all
concentration
1 mg/mL
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
wet ice
storage temp.
−20°C
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1 of 4
This Item | F2426 | F4049 | M8823 |
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form - | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| biological source mouse | biological source - | biological source mouse | biological source - |
| species reactivity all | species reactivity - | species reactivity all | species reactivity - |
| conjugate unconjugated | conjugate - | conjugate FITC conjugate | conjugate magnetic beads |
| clone M2, monoclonal | clone M2, monoclonal | clone M2, monoclonal | clone M2, monoclonal |
| form buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) | form - | form buffered aqueous solution | form suspension |
General description
The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.
Method of purification - Affinity tag purification
Method of purification - Affinity tag purification
Specificity
Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Immunogen
FLAG; peptide sequence DYKDDDDK
Application
For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting[1], immunoprecipitation (IP) [2][3][4], immunohistochemisty, immunofluorescence[5] and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Physical form
Contains 50% glycerol in phosphate buffered saline.
Preparation Note
Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Is this Ab suitable for ELISA?
1 answer-
Helpful?
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Bonjour, il est indiqué que cet anticorps marche en immunohistochimie. Quel protocole d'antigen retrieval faut-il utiliser et quelle concentration d'anticorps sur coupe FFPE? Merci
1 answer-
Please see the reference at the link below using this antibody at 1:50 dilution in IHC with antigen retrieval in citrate buffer for 5 minutes.
https://www.nature.com/articles/s41467-019-12187-5.pdfHelpful?
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モノクローナル抗FLAG® M2抗体 マウス宿主抗体(F1804)を購入しました。WBで使用しますが、抗体の推奨の希釈率、希釈液を知りたいです。よろしくお願いいたします。
1 answer-
An antibody concentration of 1 ug/mL or 1:1000 dilution is recommended for use in western blot. Please see the link below to review the product datasheet which includes the full protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdfHelpful?
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