This product is produced through bacterial fermentation. The process is generally considered to be of organic origin in the biochemical sense, as it is produced by living organisms (bacteria) rather than through purely chemical synthesis.
G7513
L-Glutamine solution
200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
L-Glutamine solution
Synonym(s):
Glavamin, Levoglutamide
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.75
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sterility
sterile-filtered
Quality Level
product line
BioXtra
form
solution
concentration
200 mM
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
SMILES string
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
Gene Information
rat ... Ggt1(116568)
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This Item | G2150 | G3126 | 59202C |
|---|---|---|---|
| form solution | form solution | form powder | form solution |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| product line BioXtra | product line - | product line ReagentPlus® | product line - |
| storage temp. −20°C | storage temp. −20°C | storage temp. room temp | storage temp. −20°C |
| impurities endotoxin, tested | impurities endotoxin, tested | impurities ≤0.2% free ammonia., ~0.5% Glutamic acid | impurities - |
| Quality Level 300, 400 | Quality Level 300 | Quality Level 200 | Quality Level 400 |
General description
L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Application
L-glutamine is an essential amino acid that is a crucial component of culture media, which serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use.
Preparation Note
Prepared in cell culture grade water.
comparable product
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Chromatograms
application for HPLC
application for HPLC
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Guten Tag, ist das L-Glutamin biologischen Ursprungs? Danke und VG!
1 answer-
Helpful?
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How much of Product G7513 should be used in cell culture media?
1 answer-
In general, MEM typically contains 0.292 g/L L-Glutamine. A 200mM Glutamine solution is equivalent to 29.23 mg/mL. So it needs to be diluted around 100 times.
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
1 answer-
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?
1 answer-
L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled. After thawing, it will be an opalescent solution. If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization. To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed. This will expose the product to the minimal amount of warming cycles.
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