Merck
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SRP0177

Sigma-Aldrich

Histone H3 full length human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

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Synonym(s):
HIST1H3E, Histone H3.1
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

15.4 kDa

packaging

pkg of 1 mg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_003532

UniProt accession no.

P68431

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HIST1H3E(8353)

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Histone H3 full length human recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

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Histone H3 (2-58) human recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP0158

Histone H3 (2-58) human

biological source

human

biological source

human

biological source

human

biological source

human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥80% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

form

aqueous solution

form

aqueous solution

form

aqueous solution

form

aqueous solution

mol wt

15.4 kDa

mol wt

12.1 kDa

mol wt

14.8 kDa

mol wt

32 kDa

packaging

pkg of 1 mg

packaging

pkg of 1 mg

packaging

pkg of 1 mg

packaging

pkg of 500 μg

General description

The gene HIST1H3E (histone 3, also referred to as H3.1) is mapped to human chromosome 6p22. It is a variant of histone 3 and is also called as the replicative histone. HIST1H3E is mainly expressed in the S-phase. In the process of cellular differentiation, HIST1H3E mRNA levels goes down as cell division slows down.
Human HIST1H3E, GenBank Accession No. NM_003532, amino acids 2-137 (end) with N-terminal His-tag, MW = 15.4kDa, expressed in an Escherichia coli expression system.

Application

Histone H3 full-length human has been used as a substrate in in vitro kinase assay for Aurora-A (AURKA) kinase and Tousled-Like Kinase 2(TLK2).

Biochem/physiol Actions

Histones are important for proper organization and modification of chromatin. HIST1H3E (histone 3, also referred to as H3.1) is mainly associated with DNA synthesis during DNA replication and might also be involved in DNA repair. It is added to the chromatin at the time of DNA replication-associated chromatin assembly. During HSV-1 (herpes simplex virus 1) infection, differential movement of HIST1H3E is associated with the assembly of viral chromatin.

Physical form

Formulated in 10 mM PBS, pH 7.4, 130 mM NaCl, 10% glycerol and 1 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70°C. Note: Protein is very sensitive to freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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